Evidence map›Paper›PMID 25673778›Full record

ArticlePlant physiology2015

Galactose-depleted xyloglucan is dysfunctional and leads to dwarfism in Arabidopsis.

Yingzhen Kong, Maria J Peña, Luciana Renna, Utku Avci, Sivakumar Pattathil, Sami T Tuomivaara, Xuemei Li, Wolf-Dieter Reiter, Federica Brandizzi, Michael G Hahn and 3 more

Open access · bronzeAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 45 papers.

0numbers the graph read from it
0cells of the map it votes in
45citing papers in PubMed
16.6field-weighted citation impact, top 1% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

45 citing papers in PubMed, 94 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Review
  5. Structure and growth of plant cell walls.Nature reviews. Molecular cell biology · 2024
    Review
  6. Article
  7. Article
  8. Article
  9. Article
  10. Article
  11. Review
  12. Article
  13. Building an extensible cell wall.Plant physiology · 2022
    Article
  14. Article
  15. Review
  16. Article
  17. Article
  18. Article
  19. Article
  20. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

13 authors at 5 institutions in 2 countries.

Yingzhen KongComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Maria J PeñaComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Luciana RennaComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Utku AvciComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Sivakumar PattathilComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Sami T TuomivaaraComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Xuemei LiComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Wolf-Dieter ReiterComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Federica BrandizziComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Michael G HahnComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Alan G DarvillComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
William S YorkComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.).
Malcolm A O'NeillComplex Carbohydrate Research Center (Y.K., M.J.P., U.A., S.P., S.T.T., M.G.H., A.G.D., W.S.Y., M.A.O.), Department of Plant Biology (M.G.H.), and Department of Biochemistry and Molecular Biology (A.G.D., W.S.Y.), University of Georgia, Athens, Georgia 30602;Tobacco Research Institute, Chinese Academy of Agricultural Sciences, Qingdao, Shandong 266101, China (Y.K.);United States Department of Energy Plant Research Laboratory (L.R., F.B.) and United States Department of Energy Great Lakes Bioenergy Research Center (F.B.), Michigan State University, East Lansing, Michigan 48824; andDepartment of Molecular and Cell Biology, University of Connecticut, Storrs, Connecticut 06269 (X.L., W.-D.R.) mao@ccrc.uga.edu.
University of Connecticut · USUniversity of Georgia · USChinese Academy of Agricultural Sciences · CNGreat Lakes Bioenergy Research Center · USUnited States Department of Energy · US

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Xyloglucan is a polysaccharide that has important roles in the formation and function of the walls that surround growing land plant cells. Many of these plants synthesize xyloglucan that contains galactose in two different side chains (L and F), which exist in distinct molecular environments. However, little is known about the contribution of these side chains to xyloglucan function. Here, we show that Arabidopsis (Arabidopsis thaliana) mutants devoid of the F side chain galactosyltransferase MURUS3 (MUR3) form xyloglucan that lacks F side chains and contains much less galactosylated xylose than its wild-type counterpart. The galactose-depleted xyloglucan is dysfunctional, as it leads to mutants that are dwarfed with curled rosette leaves, short petioles, and short inflorescence stems. Moreover, cell wall matrix polysaccharides, including xyloglucan and pectin, are not properly secreted and instead accumulate within intracellular aggregates. Near-normal growth is restored by generating mur3 mutants that produce no detectable amounts of xyloglucan. Thus, cellular processes are affected more by the presence of the dysfunctional xyloglucan than by eliminating xyloglucan altogether. To identify structural features responsible for xyloglucan dysfunction, xyloglucan structure was modified in situ by generating mur3 mutants that lack specific xyloglucan xylosyltransferases (XXTs) or that overexpress the XYLOGLUCAN L-SIDE CHAIN GALACTOSYLTRANSFERASE2 (XLT2) gene. Normal growth was restored in the mur3-3 mutant overexpressing XLT2 and in mur3-3 xxt double mutants when the dysfunctional xyloglucan was modified by doubling the amounts of galactosylated side chains. Our study assigns a role for galactosylation in normal xyloglucan function and demonstrates that altering xyloglucan side chain structure disturbs diverse cellular and physiological processes.

Indexed as

ArabidopsisArabidopsis ProteinsCell WallGalactoseGalactosyltransferasesGlucansInflorescenceMutationPectinsPhenotypePlant LeavesPlant StemsPolysaccharidesXylansArabidopsis ProteinsGalactoseGalactosyltransferasesGlucansMUR3 protein, ArabidopsisN-acetyllactosaminide alpha-1,3-galactosyltransferasePectinsPolysaccharidesXLT2 protein, ArabidopsisXylansxyloglucan

Identifiers

PMID25673778
PMCPMC4378170
OpenAlexW2100994725

What OpenQuestion holds

Textmetadata
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.