ArticleToxicological sciences : an official journal of the Society of Toxicology2015
The effects on bronchial epithelial mucociliary cultures of coarse, fine, and ultrafine particulate matter from an underground railway station.
Abstract read
In one paragraph
Article in Toxicological sciences : an official journal of the Society of Toxicology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
0numbers the graph read from it
0cells of the map it votes in
27citing papers in PubMed
–field-weighted citation impact
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
27 citing papers in PubMed.
- Novel Approach to Assess the Toxicity of PMACS ES&T air · 2026Article
- Metal and oxidative potential exposure through particle inhalation and oxidative stress biomarkers: a 2-week pilot prospective study among Parisian subway workers.International archives of occupational and environmental health · 2024Article
- Cardiolipin Membranes Promote CytochromeMolecules (Basel, Switzerland) · 2024Article
- Magnetic and microscopic investigation of airborne iron oxide nanoparticles in the London Underground.Scientific reports · 2022Article
- Adverse effects of air pollution-derived fine particulate matter on cardiovascular homeostasis and disease.Trends in cardiovascular medicine · 2022Review
- RNA-Seq Analysis of UPM-Exposed Epithelium Co-Cultivated with Macrophages and Dendritic Cells in Obstructive Lung Diseases.International journal of molecular sciences · 2022Article
- Role of air pollutants in airway epithelial barrier dysfunction in asthma and COPD.European respiratory review : an official journal of the European Respiratory Society · 2022Review
- Forgotten but not gone: Particulate matter as contaminations of mucosal systems.Biophysics reviews · 2021Review
- Interactions of nasal epithelium with macrophages and dendritic cells variously alter urban PM-induced inflammation in healthy, asthma and COPD.Scientific reports · 2021Article
- Application of green tea catechins, polysaccharides, and flavonol prevent fine dust induced bronchial damage by modulating inflammation and airway cilia.Scientific reports · 2021Article
- Effect of concentration and duration of particulate matter exposure on the transcriptome and DNA methylome of bronchial epithelial cells.Environmental epigenetics · 2021Article
- PMEnvironment international · 2020Article
- The Role and Potential Pathogenic Mechanism of Particulate Matter in Childhood Asthma: A Review and Perspective.Journal of immunology research · 2020Review
- Oxidative Stress From Exposure to the Underground Space Environment.Frontiers in public health · 2020Review
- Impacts of Organomodified Nanoclays and Their Incinerated Byproducts on Bronchial Cell Monolayer Integrity.Chemical research in toxicology · 2019Article
- Seasonal Variation in the Biological Effects of PMInternational journal of molecular sciences · 2019Article
- Particulate matter and the airway epithelium: the special case of the underground?European respiratory review : an official journal of the European Respiratory Society · 2019Article
- Mixture Effects of Diesel Exhaust and Metal Oxide Nanoparticles in Human Lung A549 Cells.Nanomaterials (Basel, Switzerland) · 2019Article
- Effects of Different Components of PMInternational journal of environmental research and public health · 2019Article
- Health effects of particulate matter air pollution in underground railway systems - a critical review of the evidence.Particle and fibre toxicology · 2019Review
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
11 authors.
Matthew Loxham*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands m.loxham@soton.ac.uk.
Rebecca J Morgan-Walsh*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Matthew J Cooper*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Cornelia Blume*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Emily J Swindle*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Patrick W Dennison*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Peter H Howarth*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Flemming R Cassee*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Damon A H Teagle*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Martin R Palmer*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands.
Donna E Davies*Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United Kingdom, NIHR Southampton Respiratory Biomedical Research Unit, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Centre for Sustainability, Environment, and Health, National Institute for Public Health and the Environment (RIVM), 3720BA Bilthoven, The Netherlands and Institute for Risk Assessment Sciences (IRAS), Utrecht University, 3508TC Utrecht, The Netherlands *Academic Unit of Clinical and Experimental Sciences, University of Southampton Faculty of Medicine, University Hospital Southampton, Southampton SO16 6YD, United Kingdom, Institute for Life Sciences, Highfield Campus, University of Southampton, Southampton SO17 1BJ, United Kingdom, Ocean and Earth Science, National Oceanography Centre Southampton, University of Southampton, Southampton SO14 3ZH, United K
Funding
Medical Research Council G0900453Wellcome Trust
Abstract
We have previously shown that underground railway particulate matter (PM) is rich in iron and other transition metals across coarse (PM10-2.5), fine (PM2.5), and quasi-ultrafine (PM0.18) fractions and is able to generate reactive oxygen species (ROS). However, there is little knowledge of whether the metal-rich nature of such particles exerts toxic effects in mucus-covered airway epithelial cell cultures or whether there is an increased risk posed by the ultrafine fraction. Monolayer and mucociliary air-liquid interface (ALI) cultures of primary bronchial epithelial cells (PBECs) were exposed to size-fractionated underground railway PM (1.1-11.1 µg/cm(2)) and release of lactate dehydrogenase and IL-8 was assayed. ROS generation was measured, and the mechanism of generation studied using desferrioxamine (DFX) and N-acetylcysteine (NAC). Expression of heme oxygenase-1 (HO-1) was determined by RT-qPCR. Particle uptake was studied by transmission electron microscopy. Underground PM increased IL-8 release from PBECs, but this was diminished in mucus-secreting ALI cultures. Fine and ultrafine PM generated a greater level of ROS than coarse PM. ROS generation by ultrafine PM was ameliorated by DFX and NAC, suggesting an iron-dependent mechanism. Despite the presence of mucus, ALI cultures displayed increased HO-1 expression. Intracellular PM was observed within vesicles, mitochondria, and free in the cytosol. The results indicate that, although the mucous layer appears to confer some protection against underground PM, ALI PBECs nonetheless detect PM and mount an antioxidant response. The combination of increased ROS-generating ability of the metal-rich ultrafine fraction and ability of PM to penetrate the mucous layer merits further research.
Indexed as
TransportationBronchiCells, CulturedCiliaEpithelial CellsHumansParticle SizeParticulate MatterParticulate Matterbronchial epitheliumenvironmental exposuremetalsparticulate matterprimary cell cultureunderground railway
Identifiers
What OpenQuestion holds
Textmetadata
LicenceCC BY
Read underepoch 390
Registered trials
None linked
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.