ArticleSensors (Basel, Switzerland)2015
Fluorescence spectroscopy approaches for the development of a real-time organophosphate detection system using an enzymatic sensor.
Article in Sensors (Basel, Switzerland), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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17 citing papers in PubMed, 51 citations in OpenAlex.
- Review
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- Use of biosensors for rapid and sensitive detection of pesticides in food samples for food safety chemical risk assessment.EFSA journal. European Food Safety Authority · 2022Article
- Monitoring of pesticide amount in fruit and vegetables by a fluorescence-based sensor.EFSA journal. European Food Safety Authority · 2022Article
- A 3D printable adapter for solid-state fluorescence measurements: the case of an immobilized enzymatic bioreceptor for organophosphate pesticides detection.Analytical and bioanalytical chemistry · 2022Article
- A FRET Approach to Detect Paraoxon among Organophosphate Pesticides Using a Fluorescent Biosensor.Sensors (Basel, Switzerland) · 2022Article
- Point-of-Care Diagnostics of COVID-19: From Current Work to Future Perspectives.Sensors (Basel, Switzerland) · 2020Review
- Highly Sensitive Detection of Chemically Modified Thio-Organophosphates by an Enzymatic Biosensing Device: An Automated Robotic Approach.Sensors (Basel, Switzerland) · 2020Article
- A review on colorimetric methods for determination of organophosphate pesticides using gold and silver nanoparticles.Mikrochimica acta · 2020Review
- Zirconia nanocomposites with carbon and iron(iii) oxide for voltammetric detection of sub-nanomolar levels of methyl parathion.Nanoscale advances · 2019Article
- Determination of Picomolar Concentrations of Paraoxon in Human Urine by Fluorescence-Based Enzymatic Assay.Sensors (Basel, Switzerland) · 2019Article
- Innovative Biocatalysts as Tools to Detect and Inactivate Nerve Agents.Scientific reports · 2018Article
- High yield production and purification of two recombinant thermostable phosphotriesterase-like lactonases from Sulfolobus acidocaldarius and Sulfolobus solfataricus useful as bioremediation tools and bioscavengers.BMC biotechnology · 2018Article
- Detection of contaminants in water supply: A review on state-of-the-art monitoring technologies and their applications.Sensors and actuators. B, Chemical · 2018Review
- Total Fluorescence Fingerprinting of Pesticides: A Reliable Approach for Continuous Monitoring of Soils and Waters.Journal of fluorescence · 2017Article
- Boosted large-scale production and purification of a thermostable archaeal phosphotriesterase-like lactonase for organophosphate decontamination.Journal of industrial microbiology & biotechnology · 2017Article
- Biochemical strategies for the detection and detoxification of toxic chemicals in the environment.World journal of biological chemistry · 2017Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Organophosphates are organic substances that contain a phosphoryl or a thiophosphoryl bond. They are mainly used around the world as pesticides, but can also be used as chemical warfare agents. Their detection is normally entrusted to techniques like GC- and LC-MS that, although sensitive, do not allow their identification on site and in real time. We have approached their identification by exploiting the high-affinity binding of these compounds with the esterase 2 from Alicyclobacillus acidocaldarius. Using an in silico analysis to evaluate the binding affinities of the enzyme with organophosphate inhibitors, like paraoxon, and other organophosphate compounds, like parathion, chlorpyriphos, and other organophosphate thio-derivatives, we have designed fluorescence spectroscopy experiments to study the quenching of the tryptophan residues after esterase 2 binding with the organophosphate pesticides. The changes in the fluorescence signals permitted an immediate and quantitative identification of these compounds from nano- to picomolar concentrations. A fluorescence based polarity-sensitive probe (ANS) was also employed as a means to understand the extent of the interactions involved, as well as to explore other ways to detect organophosphate pesticides. Finally, we designed a framework for the development of a biosensor that exploits fluorescence technology in combination with a sensitive and very stable bio-receptor.
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