ArticlePloS one2015
Cytochrome P450-dependent metabolism of caffeine in Drosophila melanogaster.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
22 citing papers in PubMed, 54 citations in OpenAlex.
- Taxonomic context and genomic architecture jointly shape expression divergence across animals.Evolution; international journal of organic evolution · 2026Article
- Identification ofFrontiers in immunology · 2026Review
- Taxonomic context and genomic architecture jointly shape expression divergence across animals.bioRxiv : the preprint server for biology · 2025Article
- Galleria mellonella as a drug discovery model to study oxidative stress.Scientific reports · 2025Article
- Transfer of Caffeine and Its Major Metabolites to Chicken Eggs.Animals : an open access journal from MDPI · 2024Article
- The effects of methylphenidate and atomoxetine on Drosophila brain at single-cell resolution and potential drug repurposing for ADHD treatment.Molecular psychiatry · 2024Article
- Neural control of redox response and microbiota-triggered inflammation inFrontiers in immunology · 2023Article
- Caffeine Delays Ethanol-Induced Sedation inBiology · 2022Article
- Developmental transcriptomics throughout the embryonic developmental process of Rhipicephalus turanicus reveals stage-specific gene expression profiles.Parasites & vectors · 2022Article
- Powerful, efficient QTL mapping in Drosophila melanogaster using bulked phenotyping and pooled sequencing.Genetics · 2022Article
- Ecdysteroid kinase-like (EcKL) paralogs confer developmental tolerance to caffeine inCurrent research in insect science · 2022Article
- Genetic stability of Aedes aegypti populations following invasion by wMel Wolbachia.BMC genomics · 2021Article
- Bacterial challenge-associated metabolic phenotypes in Hermetia illucens defining nutritional and functional benefits.Scientific reports · 2021Article
- Identification and characterization of CYPs induced in the Drosophila antenna by exposure to a plant odorant.Scientific reports · 2021Article
- Short term transcriptional responses of P450s to phytochemicals in insects and mites.Current opinion in insect science · 2021Review
- Metabolic activation and toxicological evaluation of polychlorinated biphenyls in Drosophila melanogaster.Scientific reports · 2020Article
- Alkaline Ceramidase Mediates the Oxidative Stress Response in Drosophila melanogaster Through Sphingosine.Journal of insect science (Online) · 2019Article
- Sleep inFrontiers in physiology · 2019Review
- Review
- Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Caffeine (1, 3, 7-trimethylxanthine), an alkaloid produced by plants, has antioxidant and insecticide properties that can affect metabolism and cognition. In vertebrates, the metabolites derived from caffeine have been identified, and their functions have been characterized. However, the metabolites of caffeine in insects remain unknown. Thus, using radiolabelled caffeine, we have identified some of the primary caffeine metabolites produced in the body of Drosophila melanogaster males, including theobromine, paraxanthine and theophylline. In contrast to mammals, theobromine was the predominant metabolite (paraxanthine in humans; theophylline in monkeys; 1, 3, 7-trimethyluric acid in rodents). A transcriptomic screen of Drosophila flies exposed to caffeine revealed the coordinated variation of a large set of genes that encode xenobiotic-metabolizing proteins, including several cytochromes P450s (CYPs) that were highly overexpressed. Flies treated with metyrapone--an inhibitor of CYP enzymes--showed dramatically decreased caffeine metabolism, indicating that CYPs are involved in this process. Using interference RNA genetic silencing, we measured the metabolic and transcriptomic effect of three candidate CYPs. Silencing of CYP6d5 completely abolished theobromine synthesis, whereas CYP6a8 and CYP12d1 silencing induced different consequences on metabolism and gene expression. Therefore, we characterized several metabolic products and some enzymes potentially involved in the degradation of caffeine. In conclusion, this pioneer approach to caffeine metabolism in insects opens novel perspectives for the investigation of the physiological effects of caffeine metabolites. It also indicates that caffeine could be used as a biomarker to evaluate CYP phenotypes in Drosophila and other insects.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.