ArticleF1000Research2014
Binding of a fluorescence reporter and a ligand to an odorant-binding protein of the yellow fever mosquito, Aedes aegypti.
Article in F1000Research, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 19 papers.
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Who cites it
19 citing papers in PubMed, 45 citations in OpenAlex.
- Chemosensory protein 3 is a brain host factor for the induction of enhanced-locomotory activity in the BmNPV-silkworm infection model.PLoS pathogens · 2025Article
- Impact of elevated COScientific reports · 2025Article
- Differentiation of action mechanisms between natural and synthetic repellents through neuronal electroantennogram and proteomic in Aedes aegypti (Diptera: Culicidae).Scientific reports · 2022Article
- An odorant-binding protein in the elephant's trunk is finely tuned to sex pheromone (Z)-7-dodecenyl acetate.Scientific reports · 2022Article
- Review
- Reverse Chemical Ecology Suggests Putative Primate Pheromones.Molecular biology and evolution · 2022Article
- Mammalian derived lipocalin and secretoglobin respiratory allergens strongly bind ligands with potentially immune modulating properties.Frontiers in allergy · 2022Article
- Regulation of the antennal transcriptome of the dengue vector, Aedes aegypti, during the first gonotrophic cycle.BMC genomics · 2021Article
- Olfaction-Related Gene Expression in the Antennae of Female Mosquitoes From CommonFrontiers in physiology · 2021Article
- Crystal structure of Epiphyas postvittana pheromone binding protein 3.Scientific reports · 2020Article
- Design of a Repellent Against Aedes aegypti (Diptera: Culicidae) Using in silico Simulations With AaegOBP1 Protein.Journal of medical entomology · 2020Article
- Sex and age modulate antennal chemosensory-related genes linked to the onset of host seeking in the yellow-fever mosquito, Aedes aegypti.Scientific reports · 2019Article
- A death pheromone, oleic acid, triggers hygienic behavior in honey bees (Apis mellifera L.).Scientific reports · 2018Article
- Molecular and Functional Characterization of Odorant Binding Protein 7 From the Oriental Fruit MothFrontiers in physiology · 2018Article
- Reverse chemical ecology: Olfactory proteins from the giant panda and their interactions with putative pheromones and bamboo volatiles.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- Identification and tissue expression profiling of odorant binding protein genes in the red palm weevil, Rhynchophorus ferrugineus.SpringerPlus · 2016Article
- Conserved chemosensory proteins in the proboscis and eyes of Lepidoptera.International journal of biological sciences · 2016Article
- Selectivity of odorant-binding proteins from the southern house mosquito tested against physiologically relevant ligands.Frontiers in physiology · 2015Article
- Binding of a fluorescence reporter and a ligand to an odorant-binding protein of the yellow fever mosquito, Aedes aegypti.F1000Research · 2014Article
Corrections and comments
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Odorant-binding proteins (OBPs), also named pheromone-binding proteins when the odorant is a pheromone, are essential for insect olfaction. They solubilize odorants that reach the port of entry of the olfactory system, the pore tubules in antennae and other olfactory appendages. Then, OBPs transport these hydrophobic compounds through an aqueous sensillar lymph to receptors embedded on dendritic membranes of olfactory receptor neurons. Structures of OBPs from mosquito species have shed new light on the mechanism of transport, although there is considerable debate on how they deliver odorant to receptors. An OBP from the southern house mosquito, Culex quinquefasciatus, binds the hydrophobic moiety of a mosquito oviposition pheromone (MOP) on the edge of its binding cavity. Likewise, it has been demonstrated that the orthologous protein from the malaria mosquito binds the insect repellent DEET on a similar edge of its binding pocket. A high school research project was aimed at testing whether the orthologous protein from the yellow fever mosquito, AaegOBP1, binds DEET and other insect repellents, and MOP was used as a positive control. Binding assays using the fluorescence reporter N-phenyl-1-naphtylamine (NPN) were inconclusive. However, titration of NPN fluorescence emission in AaegOBP1 solution with MOP led to unexpected and intriguing results. Quenching was observed in the initial phase of titration, but addition of higher doses of MOP led to a stepwise increase in fluorescence emission coupled with a blue shift, which can be explained at least in part by formation of MOP micelles to house stray NPN molecules.
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Registered trials
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