ArticleThe Plant cell2015
Overexpression of plastid transketolase in tobacco results in a thiamine auxotrophic phenotype.
Article in The Plant cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 43 papers.
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Who cites it
43 citing papers in PubMed.
- Simultaneous overexpression of three enzymes of chloroplast metabolism fails to improve CO2 assimilation or biomass.Journal of experimental botany · 2026Article
- ZmTH1 Is Vital for Healthy Plant Growth and Promotes Cold/Drought Tolerance by Regulating Thiamin Diphosphate-Dependent Metabolisms in Maize.Plant biotechnology journal · 2026Article
- Transketolase-mediated erythrose-4-phosphate provides an essential source for anthocyanin biosynthesis in petunia.Horticulture research · 2025Article
- Photosynthesis: Genetic Strategies Adopted to Gain Higher Efficiency.International journal of molecular sciences · 2024Review
- Effects of Low Light after Heading on the Yield of Direct Seeding Rice and Its Physiological Response Mechanism.Plants (Basel, Switzerland) · 2023Article
- PSI Photoinhibition and Changing COAntioxidants (Basel, Switzerland) · 2023Article
- Mass screening of rice mutant populations at low COFrontiers in plant science · 2023Article
- Improving plant productivity by re-tuning the regeneration of RuBP in the Calvin-Benson-Bassham cycle.The New phytologist · 2022Review
- Uridylation and the SKI complex orchestrate the Calvin cycle of photosynthesis through RNA surveillance ofProceedings of the National Academy of Sciences of the United States of America · 2022Article
- Key factors for differential drought tolerance in two contrasting wild materials of Artemisia wellbyi identified using comparative transcriptomics.BMC plant biology · 2022Article
- B vitamin supply in plants and humans: the importance of vitamer homeostasis.The Plant journal : for cell and molecular biology · 2022Review
- Biogenic Volatile Organic Compounds and Protein Expressions ofPlants (Basel, Switzerland) · 2022Article
- Two major metabolic factors for an efficient NADP-malic enzyme type C4 photosynthesis.Plant physiology · 2022Article
- Suppression of chloroplast triose phosphate isomerase evokes inorganic phosphate-limited photosynthesis in rice.Plant physiology · 2022Article
- Review
- Mapping of dwarfing QTL of Ari1327, a semi-dwarf mutant of upland cotton.BMC plant biology · 2022Article
- Analysis ofFrontiers in plant science · 2022Article
- Reduction in chloroplastic ribulose-5-phosphate-3-epimerase decreases photosynthetic capacity in Arabidopsis.Frontiers in plant science · 2022Article
- Amelioration of plant responses to drought under elevated COPhotosynthesis research · 2021Review
- Engineered cyanobacteria with additional overexpression of selected Calvin-Benson-Bassham enzymes show further increased ethanol production.Metabolic engineering communications · 2021Article
Corrections and comments
- Erratum issuedCORRECTION.2016
Authors and funding
8 authors.
Funding
Abstract
To investigate the effect of increased plastid transketolase on photosynthetic capacity and growth, tobacco (Nicotiana tabacum) plants with increased levels of transketolase protein were produced. This was achieved using a cassette composed of a full-length Arabidopsis thaliana transketolase cDNA under the control of the cauliflower mosaic virus 35S promoter. The results revealed a major and unexpected effect of plastid transketolase overexpression as the transgenic tobacco plants exhibited a slow-growth phenotype and chlorotic phenotype. These phenotypes were complemented by germinating the seeds of transketolase-overexpressing lines in media containing either thiamine pyrophosphate or thiamine. Thiamine levels in the seeds and cotyledons were lower in transketolase-overexpressing lines than in wild-type plants. When transketolase-overexpressing plants were supplemented with thiamine or thiamine pyrophosphate throughout the life cycle, they grew normally and the seed produced from these plants generated plants that did not have a growth or chlorotic phenotype. Our results reveal the crucial importance of the level of transketolase activity to provide the precursor for synthesis of intermediates and to enable plants to produce thiamine and thiamine pyrophosphate for growth and development. The mechanism determining transketolase protein levels remains to be elucidated, but the data presented provide evidence that this may contribute to the complex regulatory mechanisms maintaining thiamine homeostasis in plants.
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