ArticleScientific reports2015
A high-resolution 2D J-resolved NMR detection technique for metabolite analyses of biological samples.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 9 papers.
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Who cites it
9 citing papers in PubMed.
- Live Metabolomics with NMR.Analytical chemistry · 2026Review
- Extent of N-Terminus Folding of Semenogelin 1 Cleavage Product Determines Tendency to Amyloid Formation.International journal of molecular sciences · 2023Article
- Advances in Microbial NMR Metabolomics.Advances in experimental medicine and biology · 2023Article
- NMR Spectroscopy Applied to the Metabolic Analysis of Natural Extracts ofMolecules (Basel, Switzerland) · 2022Article
- Studying Metabolism by NMR-Based Metabolomics.Frontiers in molecular biosciences · 2022Review
- Identification of antiplasmodial triterpenes from Keetia species using NMR-based metabolic profiling.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- An Overview of Metabolic Phenotyping in Blood Pressure Research.Current hypertension reports · 2018Review
- Bacterial Substrate Transformation Tracked by Stable-Isotope-Guided NMR Metabolomics: Application in a Natural Aquatic Microbial Community.Metabolites · 2017Article
- Identification of Gastric Cancer Biomarkers Using 1H Nuclear Magnetic Resonance Spectrometry.PloS one · 2016Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
NMR spectroscopy is a commonly used technique for metabolite analyses. Due to the observed macroscopic magnetic susceptibility in biological tissues, current NMR acquisitions in measurements of biological tissues are generally performed on tissue extracts using liquid NMR or on tissues using magic-angle spinning techniques. In this study, we propose an NMR method to achieve high-resolution J-resolved information for metabolite analyses directly from intact biological samples. A dramatic improvement in spectral resolution is evident in our contrastive demonstrations on a sample of pig brain tissue. Metabolite analyses for a postmortem fish from fresh to decayed statuses are presented to further reveal the capability of the proposed method. This method is a previously-unreported high-resolution 2D J-resolved spectroscopy for biological applications without specialised hardware requirements or complicated sample pretreatments. It provides a significant contribution to metabolite analyses of biological samples, and may be potentially applicable to in vivo samples. Furthermore, this method also can be applied to measurements of semisolid and viscous samples.
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