ArticleDevelopmental cell2015
E2a is necessary for Smad2/3-dependent transcription and the direct repression of lefty during gastrulation.
Article in Developmental cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 15 papers.
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Who cites it
15 citing papers in PubMed, 25 citations in OpenAlex.
- A prevalent disease-associated SNP in the humanNAR molecular medicine · 2026Article
- PIAS4 regulates pluripotency exit and cell fate commitment in porcine embryonic stem cells.Fundamental research · 2025Article
- Quantitative analysis of transcriptome dynamics provides novel insights into developmental state transitions.BMC genomics · 2022Article
- Nodal and <i>churchill1</i> position the expression of a notch ligand during <i>Xenopus</i> germ layer segregation.Life science alliance · 2022Article
- Uncovering the mesendoderm gene regulatory network through multi-omic data integration.Cell reports · 2022Article
- T-Cell Factors as Transcriptional Inhibitors: Activities and Regulations in Vertebrate Head Development.Frontiers in cell and developmental biology · 2021Review
- E2A regulates neural ectoderm fate specification in human embryonic stem cells.Development (Cambridge, England) · 2020Article
- The transcription factor E2A drives neural differentiation in pluripotent cells.Development (Cambridge, England) · 2020Article
- Morpholinos Do Not Elicit an Innate Immune Response during Early Xenopus Embryogenesis.Developmental cell · 2019Review
- TGF-β Family Signaling in Early Vertebrate Development.Cold Spring Harbor perspectives in biology · 2018Review
- Different murine-derived feeder cells alter the definitive endoderm differentiation of human induced pluripotent stem cells.PloS one · 2018Article
- Hepatocyte Nuclear Factor 4 Alpha Promotes Definitive Endoderm Differentiation from Human Induced Pluripotent Stem Cells.Stem cell reviews and reports · 2017Article
- Id genes are essential for early heart formation.Genes & development · 2017Article
- A catalog of Xenopus tropicalis transcription factors and their regional expression in the early gastrula stage embryo.Developmental biology · 2017Article
- Keeping a lid on nodal: transcriptional and translational repression of nodal signalling.Open biology · 2016Review
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Authors and funding
2 authors at 1 institution in 1 country.
Funding
Abstract
Transcription factor complexes have varied effects on cell fate and behavior, but how this diversification of function occurs is largely unknown. The Nodal signaling pathway has many biological functions that all converge on the transcription factors Smad2/3. Smad2/3 has many cofactors, and alternative usage of these may provide a mechanism for modulating Smad2/3 function. Here, we investigate how perturbation of the cofactor E2a affects global patterns of Smad2/3 binding and gene expression during gastrulation. We find that E2a regulates early development in two ways. E2a changes the position of Smad2/3 binding at the Nodal inhibitor lefty, resulting in direct repression of lefty that is critical for mesendoderm specification. Separately, E2a is necessary to drive transcription of Smad2/3 target genes, including critical regulators of dorsal cell fate and morphogenesis. Overall, we find that E2a functions as both a transcriptional repressor and activator to precisely regulate Nodal signaling.
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