ArticleNature communications2015
Imaging single cells in a beam of live cyanobacteria with an X-ray laser.
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Article in Nature communications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 64 papers.
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64citing papers in PubMed
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64 citing papers in PubMed.
- Biological applications at the AQUA beamline of the EuPRAXIA@SPARC_LAB free electron laser.European biophysics journal : EBJ · 2026Article
- Impact of gas background on XFEL single-particle imaging.Scientific reports · 2025Article
- Coaxial helium electrospray for single-particle imaging at X-ray free electron lasers.Journal of synchrotron radiation · 2025Article
- Macromolecular crystallography and biology at the Linac Coherent Light Source.Journal of synchrotron radiation · 2025Article
- Dynamic Coherent Diffractive Imaging with Modulus Enforced Probe and Low Spatial Frequency Constraints.Sensors (Basel, Switzerland) · 2025Article
- Non-linear enhancement of ultrafast X-ray diffraction through transient resonances.Nature communications · 2025Article
- Aerosol size determination via light scattering of viruses and protein complexes.Communications physics · 2025Article
- Review
- SPRING, an effective and reliable framework for image reconstruction in single-particle Coherent Diffraction Imaging.npj computational materials · 2025Article
- Coherent X-ray diffraction imaging of single particles: background impact on 3D reconstruction.Journal of applied crystallography · 2024Article
- Development of the multiplex imaging chamber at PAL-XFEL.Journal of synchrotron radiation · 2024Article
- Enhancing electrospray ionization efficiency for particle transmission through an aerodynamic lens stack.Journal of synchrotron radiation · 2024Article
- Helium-electrospray improves sample delivery in X-ray single-particle imaging experiments.Scientific reports · 2024Article
- Observation of a single protein by ultrafast X-ray diffraction.Light, science & applications · 2024Article
- Structure determination using high-order spatial correlations in single-particle X-ray scattering.IUCrJ · 2024Article
- Protocol using similarity score and improved shrink-wrap algorithm for better convergence of phase-retrieval calculation in X-ray diffraction imaging.Journal of synchrotron radiation · 2024Article
- Similarity score for screening phase-retrieved maps in X-ray diffraction imaging - characterization in reciprocal space.Journal of synchrotron radiation · 2024Article
- In situ label-free X-ray imaging for visualizing the localization of nanomedicines and subcellular architecture in intact single cells.Nature protocols · 2024Review
- Micropipette aspiration as a tool for single-particle X-ray imaging and diffraction.Journal of synchrotron radiation · 2023Article
- Subcellular omics: a new frontier pushing the limits of resolution, complexity and throughput.Nature methods · 2023Article
4 more citing papers are in PubMed but not listed here.
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Authors and funding
39 authors.
Gijs van der SchotLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Martin SvendaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Filipe R N C Maia1] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden [2] Lawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943-256, Berkeley, California 94720, USA.
Max HantkeLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Daniel P DePonte1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
M Marvin Seibert1] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden [2] LCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Andrew Aquila1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] The European XFEL GmbH, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
Joachim Schulz1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] The European XFEL GmbH, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
Richard KirianCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Mengning LiangCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Francesco Stellato1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] I.N.F.N. and Physics Department, University of Rome 'Tor Vergata', Via della Ricerca Scientifica 1, 00133 Rome, Italy.
Bianca IwanLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Jakob AndreassonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Nicusor TimneanuLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.ORCID 0000000173280400
Daniel WestphalLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
F Nunes AlmeidaLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Dusko OdicLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Dirk HasseLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Gunilla H CarlssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Daniel S D LarssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Anton BartyLawrence Berkeley National Lab, 1 Cyclotron Road Mail Stop 943-256, Berkeley, California 94720, USA.
Andrew V Martin1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] ARC Centre of Excellence for Coherent X-ray Science, School of Physics, University of Melbourne, Parkville, Victoria 3010, Australia.
Sebastian SchorbLCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Christoph BostedtLCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
John D BozekLCLS, SLAC National Accelerator Laboratory, 2575 Sand Hill Road, Menlo Park, California 94025, USA.
Daniel RollesCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Artem Rudenko1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] Department of Physics, Kansas State University, 331 Cardwell Hall, Manhattan, Kansas 66506, USA.
Sascha EppCenter for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany.
Lutz FoucarMax-Planck-Institut für medizinische Forschung, Jahnstr. 29, 69120 Heidelberg, Germany.
Benedikt RudekPhysikalisch-Technische Bundesanstalt (PTB) Bundesallee 100, 38116 Braunschweig, Germany.
Robert HartmannPNSensor GmbH, Römerstrasse 28, 80803 München, Germany.
Nils Kimmel1] PNSensor GmbH, Römerstrasse 28, 80803 München, Germany [2] Max Planck Institute for Extraterrestrial Physics, Giessenbachstrasse, 85741 Garching, Germany.
Peter HollPNSensor GmbH, Römerstrasse 28, 80803 München, Germany.
Lars EnglertInstitute of Physics, Carl von Ossietzky Universitaet Oldenburg, Carl-von-Ossietzky-Straße 9-11, D-26129 Oldenburg, Germany.
Ne-Te Duane LohCentre for BioImaging Sciences, National University of Singapore, 14 Science Drive 4, Singapore 117557, Singapore.
Henry N Chapman1] Center for Free-Electron Laser Science, DESY, Notkestrasse 85, 22607 Hamburg, Germany [2] University of Hamburg, Notkestrasse 85, 22607 Hamburg, Germany.ORCID 0000000246551743
Inger AnderssonLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Janos Hajdu1] Laboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden [2] The European XFEL GmbH, Albert-Einstein-Ring 19, 22761 Hamburg, Germany.
Tomas EkebergLaboratory of Molecular Biophysics, Department of Cell and Molecular Biology, Uppsala University, Husargatan 3 (Box 596), SE-751 24 Uppsala, Sweden.
Funding
No grant is acknowledged in the PubMed record.
Abstract
There exists a conspicuous gap of knowledge about the organization of life at mesoscopic levels. Ultra-fast coherent diffractive imaging with X-ray free-electron lasers can probe structures at the relevant length scales and may reach sub-nanometer resolution on micron-sized living cells. Here we show that we can introduce a beam of aerosolised cyanobacteria into the focus of the Linac Coherent Light Source and record diffraction patterns from individual living cells at very low noise levels and at high hit ratios. We obtain two-dimensional projection images directly from the diffraction patterns, and present the results as synthetic X-ray Nomarski images calculated from the complex-valued reconstructions. We further demonstrate that it is possible to record diffraction data to nanometer resolution on live cells with X-ray lasers. Extension to sub-nanometer resolution is within reach, although improvements in pulse parameters and X-ray area detectors will be necessary to unlock this potential.
Indexed as
LasersAerosolsCyanobacteriaData AccuracyElectronsImaging, Three-DimensionalInjectionsOptical PhenomenaPhotonsSingle-Cell AnalysisX-Ray DiffractionX-RaysAerosols
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PMID
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