ArticleArchives of biochemistry and biophysics2015
The invertebrate Caenorhabditis elegans biosynthesizes ascorbate.
Article in Archives of biochemistry and biophysics, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 16 papers.
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Who cites it
16 citing papers in PubMed, 28 citations in OpenAlex.
- Combined metallomics and metabolomics reveal impact of metal homeostasis on biological pathways in C. elegans.Analytical and bioanalytical chemistry · 2026Article
- Exogenous 2-Keto-L-Gulonic Acid Supplementation Promotes L-Ascorbic Acid Biosynthesis inInternational journal of molecular sciences · 2026Article
- Cecytb-2, a CytochromeBiomolecules · 2025Article
- C11orf54 catalyzes L-xylulose formation in human metabolism.Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- L-gulono-γ-lactone Oxidase, the Key Enzyme for L-Ascorbic Acid Biosynthesis.Current issues in molecular biology · 2024Review
- UPReLife · 2024Article
- Dietary supplementation with probiotics promotes weight loss by reshaping the gut microbiome and energy metabolism in obese dogs.Microbiology spectrum · 2024Article
- Advances in Antioxidant Applications for CombatingToxics · 2023Review
- Review
- The evolution of vitamin C biosynthesis and transport in animals.BMC ecology and evolution · 2022Article
- Article
- Inhibition of lipid peroxidation during the reproductive period extends the lifespan ofJournal of clinical biochemistry and nutrition · 2020Article
- Multiple independent L-gulonolactone oxidase (GULO) gene losses and vitamin C synthesis reacquisition events in non-Deuterostomian animal species.BMC evolutionary biology · 2019Article
- Ascorbic acid metabolism and functions: A comparison of plants and mammals.Free radical biology & medicine · 2018Review
- Vitamin BRedox biology · 2017Article
- Metabolome and proteome changes with aging in Caenorhabditis elegans.Experimental gerontology · 2015Article
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Authors and funding
6 authors at 1 institution in 1 country.
Funding
Abstract
l-Ascorbate, commonly known as vitamin C, serves as an antioxidant and cofactor essential for many biological processes. Distinct ascorbate biosynthetic pathways have been established for animals and plants, but little is known about the presence or synthesis of this molecule in invertebrate species. We have investigated ascorbate metabolism in the nematode Caenorhabditis elegans, where this molecule would be expected to play roles in oxidative stress resistance and as cofactor in collagen and neurotransmitter synthesis. Using high-performance liquid chromatography and gas-chromatography mass spectrometry, we determined that ascorbate is present at low amounts in the egg stage, L1 larvae, and mixed animal populations, with the egg stage containing the highest concentrations. Incubating C. elegans with precursor molecules necessary for ascorbate synthesis in plants and animals did not significantly alter ascorbate levels. Furthermore, bioinformatic analyses did not support the presence in C. elegans of either the plant or the animal biosynthetic pathway. However, we observed the complete (13)C-labeling of ascorbate when C. elegans was grown with (13)C-labeled Escherichia coli as a food source. These results support the hypothesis that ascorbate biosynthesis in invertebrates may proceed by a novel pathway and lay the foundation for a broader understanding of its biological role.
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Registered trials
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