ArticleFrontiers in microbiology2015
Listeria monocytogenes varies among strains to maintain intracellular pH homeostasis under stresses by different acids as analyzed by a high-throughput microplate-based fluorometry.
Article in Frontiers in microbiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 25 papers.
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Who cites it
25 citing papers in PubMed, 60 citations in OpenAlex.
- Review
- Determination of the combined effect of grape seed extract and cold atmospheric plasma on foodborne pathogens and their environmental stress knockout mutants.Applied and environmental microbiology · 2024Article
- Characterisation of the growth behaviour of Listeria monocytogenes in Listeria synthetic media.Environmental microbiology reports · 2023Article
- Assessment of the influence of selected stress factors on the growth and survival of Listeria monocytogenes.BMC microbiology · 2023Article
- A comprehensive investigation of protein expression profiles inFrontiers in microbiology · 2023Article
- Bacterial Surface Disturbances Affecting Cell Function during Exposure to Three-Compound Nanocomposites Based on Graphene Materials.Nanomaterials (Basel, Switzerland) · 2022Article
- Effects of Weak Acids on the Microbiological, Nutritional and Sensory Quality of Baltic Herring (Foods (Basel, Switzerland) · 2022Article
- Identification and evaluation of a panel of strong constitutive promoters in Listeria monocytogenes for improving the expression of foreign antigens.Applied microbiology and biotechnology · 2021Article
- Alternative σ Factors Regulate Overlapping as Well as Distinct Stress Response and Metabolic Functions inPathogens (Basel, Switzerland) · 2021Article
- Internal pH and Acid Anion Accumulation inFrontiers in microbiology · 2021Article
- Landscape of Stress Response and Virulence Genes AmongFrontiers in microbiology · 2021Review
- Comparative Review of the Responses ofGenes · 2020Review
- An M29 Aminopeptidase fromMicroorganisms · 2020Article
- pH-Sensitive Ratiometric Fluorescent Probe for Evaluation of Tumor Treatments.Materials (Basel, Switzerland) · 2019Article
- Assembly and Characterization of a Pathogen Strain Collection for Produce Safety Applications: Pre-growth Conditions Have a Larger Effect on Peroxyacetic Acid Tolerance Than Strain Diversity.Frontiers in microbiology · 2019Article
- Surviving host - and food relevant stresses: phenotype of L. monocytogenes strains isolated from food and clinical sources.Scientific reports · 2018Article
- Intracellular pHluorin as Sensor for Easy Assessment of Bacteriocin-Induced Membrane-Damage inFrontiers in microbiology · 2018Article
- Antimicrobial Properties and Mechanism of Action of Some Plant Extracts Against Food Pathogens and Spoilage Microorganisms.Frontiers in microbiology · 2018Article
- Carboxyl-Terminal Residues N478 and V479 Required for the Cytolytic Activity of Listeriolysin O Play a Critical Role inFrontiers in immunology · 2017Article
- Thioredoxin A Is Essential for Motility and Contributes to Host Infection ofFrontiers in cellular and infection microbiology · 2017Article
Corrections and comments
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Authors and funding
10 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Listeria monocytogenes, a food-borne pathogen, has the capacity to maintain intracellular pH (pHi) homeostasis in acidic environments, but the underlying mechanisms remain elusive. Here, we report a simple microplate-based fluorescent method to determine pHi of listerial cells that were prelabeled with the fluorescent dye carboxyfluorescein diacetate N-succinimidyl ester and subjected to acid stress. We found that L. monocytogenes responds differently among strains toward organic and inorganic acids to maintain pHi homeostasis. The capacity of L. monocytogenes to maintain pHi at extracellular pH 4.5 (pHex) was compromised in the presence of acetic acid and lactic acid, but not by hydrochloric acid and citric acid. Organic acids exhibited more inhibitory effects than hydrochloric acid at certain pH conditions. Furthermore, the virulent stains L. monocytogenes EGDe, 850658 and 10403S was more resistant to acidic stress than the avirulent M7 which showed a defect in maintaining pHi homeostasis. Deletion of sigB, a stress-responsive alternative sigma factor from 10403S, markedly altered intracellular pHi homeostasis, and showed a significant growth and survival defect under acidic conditions. Thus, this work provides new insights into bacterial survival mechanism to acidic stresses.
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