ArticlePlant physiology2015
A small phospholipase A2-α from castor catalyzes the removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds.
Article in Plant physiology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 56 citations in OpenAlex.
- Understanding the dynamic nature of plant lipid anabolic and catabolic metabolism is key to sustainable oilseed engineering.The New phytologist · 2026Review
- Comparative Metabolome and Transcriptome Analysis Reveals the Possible Roles of Rice Phospholipase A Genes in the Accumulation of Oil in Grains.International journal of molecular sciences · 2024Article
- 13C-labeling reveals non-conventional pathways providing carbon for hydroxy fatty acid synthesis in Physaria fendleri.Journal of experimental botany · 2024Article
- Biosynthesis and Transfer of α-Elostearic Acid In Vivo inInternational journal of molecular sciences · 2023Article
- Expression of Physaria longchain acyl-CoA synthetases and hydroxy fatty acid accumulation in transgenic Arabidopsis.Journal of plant physiology · 2022Article
- Enhanced production of hydroxy fatty acids in Arabidopsis seed through modification of multiple gene expression.Biotechnology for biofuels and bioproducts · 2022Article
- Dynamic Changes in Membrane Lipid Metabolism and Antioxidant Defense During Soybean (Frontiers in plant science · 2022Article
- Critical metabolic pathways and SAD/FADs, WRI1s, and DGATs cooperate for high-oleic acid oil production in developing oil tea (Horticulture research · 2022Article
- Genetic Engineering of Lesquerella with Increased Ricinoleic Acid Content in Seed Oil.Plants (Basel, Switzerland) · 2021Article
- Molecular Control of Oil Metabolism in the Endosperm of Seeds.International journal of molecular sciences · 2021Review
- Transcriptome Analysis and Identification of Lipid Genes inInternational journal of molecular sciences · 2021Article
- Castor LPCAT and PDAT1A Act in Concert to Promote Transacylation of Hydroxy-Fatty Acid onto Triacylglycerol.Plant physiology · 2020Article
- Functional Omics Identifies Serine Hydrolases That Mobilize Storage Lipids during Rice Seed Germination.Plant physiology · 2020Article
- Functional Characterization of Lysophosphatidylcholine: Acyl-CoA Acyltransferase Genes From Sunflower (Frontiers in plant science · 2020Article
- Article
- Castor patatin-like phospholipase A IIIβ facilitates removal of hydroxy fatty acids from phosphatidylcholine in transgenic Arabidopsis seeds.Plant molecular biology · 2019Article
- Tri-Hydroxy-Triacylglycerol Is Efficiently Produced by Position-Specific Castor Acyltransferases.Plant physiology · 2019Article
- Tissue-specific differences in metabolites and transcripts contribute to the heterogeneity of ricinoleic acid accumulation in Ricinus communis L. (castor) seeds.Metabolomics : Official journal of the Metabolomic Society · 2019Article
- Secretory Phospholipases AFrontiers in plant science · 2019Review
- Identification of genes associated with ricinoleic acid accumulation inBiotechnology for biofuels · 2019Article
Corrections and comments
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Authors and funding
4 authors at 2 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Ricinoleic acid, an industrially useful hydroxy fatty acid (HFA), only accumulates to high levels in the triacylglycerol fraction of castor (Ricinus communis) endosperm, even though it is synthesized on the membrane lipid phosphatidylcholine (PC) from an oleoyl ester. The acyl chains of PC undergo intense remodeling through the process of acyl editing. The identities of the proteins involved in this process, however, are unknown. A phospholipase A2 (PLA2) is thought to be involved in the acyl-editing process. We show here a role for RcsPLA2α in the acyl editing of HFA esterified to PC. RcsPLA2α was identified by its high relative expression in the castor endosperm transcriptome. Coexpression in Arabidopsis (Arabidopsis thaliana) seeds of RcsPLA2α with the castor fatty acid hydroxylase RcFAH12 led to a dramatic decrease in seed HFA content when compared with RcFAH12 expression alone in both PC and the neutral lipid fraction. The low-HFA trait was heritable and gene dosage dependent, with hemizygous lines showing intermediate HFA levels. The low seed HFA levels suggested that RcsPLA2α functions in vivo as a PLA2 with HFA specificity. Activity assays with yeast (Saccharomyces cerevisiae) microsomes showed a high specificity of RcsPLA2α for ricinoleic acid, superior to that of the endogenous Arabidopsis PLA2α. These results point to RcsPLA2α as a phospholipase involved in acyl editing, adapted to specifically removing HFA from membrane lipids in seeds.
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