Evidence map›Paper›PMID 25667270›Full record

ArticleInfection and immunity2015

Molecular analysis of asymptomatic bacteriuria Escherichia coli strain VR50 reveals adaptation to the urinary tract by gene acquisition.

Scott A Beatson, Nouri L Ben Zakour, Makrina Totsika, Brian M Forde, Rebecca E Watts, Amanda N Mabbett, Jan M Szubert, Sohinee Sarkar, Minh-Duy Phan, Kate M Peters and 13 more

Open access · bronzeAbstract readCase Reports
PubMed Full text at PMC Publisher
In one paragraph

Article in Infection and immunity, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.

0numbers the graph read from it
0cells of the map it votes in
18citing papers in PubMed
–field-weighted citation impact, top 97% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

18 citing papers in PubMed, 0 citations in OpenAlex.

  1. Article
  2. Article
  3. Article
  4. Adaptive strategies of uropathogenic Escherichia coli CFT073: from growth in lab media to virulence during host cell adhesion.International microbiology : the official journal of the Spanish Society for Microbiology · 2022
    Article
  5. Article
  6. Article
  7. Review
  8. Comparative Pathogenomics ofInfection and immunity · 2021
    Article
  9. Recurrent Urinary Tract Infection: A Mystery in Search of Better Model Systems.Frontiers in cellular and infection microbiology · 2021
    Review
  10. Article
  11. Article
  12. UniqueInternational journal of molecular sciences · 2020
    Article
  13. Review
  14. Frontiers in microbiology · 2019
    Article
  15. Review
  16. Article
  17. Article
  18. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

23 authors at 4 institutions in 3 countries.

Scott A BeatsonAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia s.beatson@uq.edu.au m.schembri@uq.edu.au.
Nouri L Ben ZakourAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Makrina TotsikaAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Brian M FordeAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Rebecca E WattsAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Amanda N MabbettAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Jan M SzubertAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Sohinee SarkarAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Minh-Duy PhanAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Kate M PetersAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Nicola K PettyAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Nabil-Fareed AlikhanAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Mitchell J SullivanAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Jayde A GawthorneAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Mitchell Stanton-CookAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Nguyen Thi Khanh NhuAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia.
Teik Min ChongDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Wai-Fong YinDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Kok-Gan ChanDivision of Genetics and Molecular Biology, Institute of Biological Sciences, Faculty of Science, University of Malaya, Kuala Lumpur, Malaysia.
Viktoria HancockCentre for Biological Sequence Analysis, DTU Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
David W UsseryCentre for Biological Sequence Analysis, DTU Department of Systems Biology, Technical University of Denmark, Lyngby, Denmark.
Glen C UlettSchool of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia Griffith Health Institute and School of Medical Science, Griffith Health Centre, Griffith University, Gold Coast, Queensland, Australia.
Mark A SchembriAustralian Infectious Diseases Research Centre, The University of Queensland, Brisbane, Queensland, Australia School of Chemistry and Molecular Biosciences, The University of Queensland, Brisbane, Queensland, Australia s.beatson@uq.edu.au m.schembri@uq.edu.au.ORCID http://orcid.org/0000-0003-4863-9260
The University of Queensland · AUUniversity of Malaya · MYTechnical University of Denmark · DKGriffith University · AU

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Urinary tract infections (UTIs) are among the most common infectious diseases of humans, with Escherichia coli responsible for >80% of all cases. One extreme of UTI is asymptomatic bacteriuria (ABU), which occurs as an asymptomatic carrier state that resembles commensalism. To understand the evolution and molecular mechanisms that underpin ABU, the genome of the ABU E. coli strain VR50 was sequenced. Analysis of the complete genome indicated that it most resembles E. coli K-12, with the addition of a 94-kb genomic island (GI-VR50-pheV), eight prophages, and multiple plasmids. GI-VR50-pheV has a mosaic structure and contains genes encoding a number of UTI-associated virulence factors, namely, Afa (afimbrial adhesin), two autotransporter proteins (Ag43 and Sat), and aerobactin. We demonstrated that the presence of this island in VR50 confers its ability to colonize the murine bladder, as a VR50 mutant with GI-VR50-pheV deleted was attenuated in a mouse model of UTI in vivo. We established that Afa is the island-encoded factor responsible for this phenotype using two independent deletion (Afa operon and AfaE adhesin) mutants. E. coli VR50afa and VR50afaE displayed significantly decreased ability to adhere to human bladder epithelial cells. In the mouse model of UTI, VR50afa and VR50afaE displayed reduced bladder colonization compared to wild-type VR50, similar to the colonization level of the GI-VR50-pheV mutant. Our study suggests that E. coli VR50 is a commensal-like strain that has acquired fitness factors that facilitate colonization of the human bladder.

Indexed as

Adaptation, BiologicalEvolution, MolecularAdultAnimalsBacterial AdhesionBacteriuriaCarrier StateCell LineDNA, BacterialEpithelial CellsEscherichia coliEscherichia coli InfectionsFemaleGenome, BacterialHumansMice, Inbred C57BLDNA, Bacterial

Identifiers

PMID25667270
PMCPMC4399054
OpenAlexW1554541227

What OpenQuestion holds

Textmetadata
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.