ArticleMolecules and cells2015
Expression of the floral repressor miRNA156 is positively regulated by the AGAMOUS-like proteins AGL15 and AGL18.
Article in Molecules and cells, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 40 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
40 citing papers in PubMed.
- Regulation of developmental transitions in plants: the role of VIVAPAROUS ABI3-LIKE (VAL) repressors.Journal of plant research · 2026Review
- Transcriptome Analysis Reveals Association of E-Class AmMADS-Box Genes with Petal Malformation inInternational journal of molecular sciences · 2025Article
- From the archives: the shape of flowers, timely flowering, and floral organ longevity.The Plant cell · 2025Article
- Insights into genetics of floral development in Amborella trichopoda Baill. through genome-wide survey and expression analysis of MADS-Box transcription factors.Scientific reports · 2025Article
- Evolution, Structural and Functional Characteristics of thePlants (Basel, Switzerland) · 2024Article
- miR156-SPL and miR169-NF-YA Modules Regulate the Induction of Somatic Embryogenesis in Arabidopsis via LEC- and Auxin-Related Pathways.International journal of molecular sciences · 2024Article
- Temporal regulation of vegetative phase change in plants.Developmental cell · 2024Review
- Nuclear Localization of G3BP6 Is Essential for the Flowering Transition inBiomolecules · 2023Article
- Flowering in sugarcane-insights from the grasses.3 Biotech · 2023Review
- Understanding the evolution of miRNA biogenesis machinery in plants with special focus on rice.Functional & integrative genomics · 2023Article
- Transcriptional regulation of oil biosynthesis in seed plants: Current understanding, applications, and perspectives.Plant communications · 2022Review
- Role of a ZF-HD Transcription Factor in miR157-Mediated Feed-Forward Regulatory Module That Determines Plant Architecture inInternational journal of molecular sciences · 2022Article
- Genome-Wide Analyses of MADS-Box Genes inPlants (Basel, Switzerland) · 2022Article
- The Characters of Non-Coding RNAs and Their Biological Roles in Plant Development and Abiotic Stress Response.International journal of molecular sciences · 2022Review
- The MADS-domain factor AGAMOUS-Like18 promotes somatic embryogenesis.Plant physiology · 2022Article
- Interactome of Arabidopsis Thaliana.Plants (Basel, Switzerland) · 2022Article
- Roles of microRNAs in abiotic stress response and characteristics regulation of plant.Frontiers in plant science · 2022Review
- Convergent evolution of the annual life history syndrome from perennial ancestors.Frontiers in plant science · 2022Review
- Cell division in the shoot apical meristem is a trigger for miR156 decline and vegetative phase transition inProceedings of the National Academy of Sciences of the United States of America · 2021Article
- VAL genes regulate vegetative phase change via miR156-dependent and independent mechanisms.PLoS genetics · 2021Article
Corrections and comments
PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.
Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The regulation of flowering time has crucial implications for plant fitness. MicroRNA156 (miR156) represses the floral transition in Arabidopsis thaliana, but the mechanisms regulating its transcription remain unclear. Here, we show that two AGAMOUS-like proteins, AGL15 and AGL18, act as positive regulators of the expression of MIR156. Small RNA northern blot analysis revealed a significant decrease in the levels of mature miR156 in agl15 agl18 double mutants, but not in the single mutants, suggesting that AGL15 and AGL18 co-regulate miR156 expression. Histochemical analysis further indicated that the double mutants showed a reduction in MIR156 promoter strength. The double mutants also showed reduced abundance of pri-miR156a and pri-miR156c, two of the primary transcripts from MIR156 genes. Electrophoretic mobility shift assays demonstrated that AGL15 directly associated with the CArG motifs in the MIR156a/c promoters. AGL18 did not show binding affinity to the CArG motifs, but pull-down and yeast two-hybrid assays showed that AGL18 forms a heterodimer with AGL15. GFP reporter assays and bimolecular fluorescence complementation (BiFC) showed that AGL15 and AGL18 co-localize in the nucleus and confirmed their in vivo interaction. Overexpression of miR156 did not affect the levels of AGL15 and AGL18 transcripts. Taking these data together, we present a model for the transcriptional regulation of MIR156. In this model, AGL15 and AGL18 may form a complex along with other proteins, and bind to the CArG motifs of the promoters of MIR156 to activate the MIR156 expression.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.