ArticleScientific reports2015
Fast super-resolution imaging with ultra-high labeling density achieved by joint tagging super-resolution optical fluctuation imaging.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers.
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Who cites it
12 citing papers in PubMed.
- Electrochemically controlled switching of dyes for enhanced superresolution optical fluctuation imaging (EC-SOFI).Proceedings of the National Academy of Sciences of the United States of America · 2025Article
- SOGO-SOFI, light-modulated super-resolution optical fluctuation imaging using only 20 raw frames for high-fidelity reconstruction.Fundamental research · 2025Article
- Advancements and Practical Considerations for Biophysical Research: Navigating the Challenges and Future of Super-resolution Microscopy.Chemical & biomedical imaging · 2024Review
- Live-Cell SOFI Correlation with SMLM and AFM Imaging.ACS bio & med chem Au · 2023Article
- Fluorescent Nanoparticles for Super-Resolution Imaging.Chemical reviews · 2022Review
- Nanoscale characterization of drug-induced microtubule filament dysfunction using super-resolution microscopy.BMC biology · 2021Article
- Systematic imaging in medicine: a comprehensive review.European journal of nuclear medicine and molecular imaging · 2021Review
- Quantum Dots for Improved Single-Molecule Localization Microscopy.The journal of physical chemistry. B · 2021Article
- Super-resolution nanoscopy by coherent control on nanoparticle emission.Science advances · 2020Article
- Nanoparticles for super-resolution microscopy and single-molecule tracking.Nature methods · 2018Review
- Sparse deconvolution of high-density super-resolution images.Scientific reports · 2016Article
- Model-free uncertainty estimation in stochastical optical fluctuation imaging (SOFI) leads to a doubled temporal resolution.Biomedical optics express · 2016Article
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Authors and funding
8 authors.
Funding
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Abstract
Previous stochastic localization-based super-resolution techniques are largely limited by the labeling density and the fidelity to the morphology of specimen. We report on an optical super-resolution imaging scheme implementing joint tagging using multiple fluorescent blinking dyes associated with super-resolution optical fluctuation imaging (JT-SOFI), achieving ultra-high labeling density super-resolution imaging. To demonstrate the feasibility of JT-SOFI, quantum dots with different emission spectra were jointly labeled to the tubulin in COS7 cells, creating ultra-high density labeling. After analyzing and combining the fluorescence intermittency images emanating from spectrally resolved quantum dots, the microtubule networks are capable of being investigated with high fidelity and remarkably enhanced contrast at sub-diffraction resolution. The spectral separation also significantly decreased the frame number required for SOFI, enabling fast super-resolution microscopy through simultaneous data acquisition. As the joint-tagging scheme can decrease the labeling density in each spectral channel, thereby bring it closer to single-molecule state, we can faithfully reconstruct the continuous microtubule structure with high resolution through collection of only 100 frames per channel. The improved continuity of the microtubule structure is quantitatively validated with image skeletonization, thus demonstrating the advantage of JT-SOFI over other localization-based super-resolution methods.
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Registered trials
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