ArticleMethods in molecular biology (Clifton, N.J.)2015
Manipulating archaeal systems to permit analyses of transcription elongation-termination decisions in vitro.
Article in Methods in molecular biology (Clifton, N.J.), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 10 papers.
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Who cites it
10 citing papers in PubMed.
- Archaeal histone-based chromatin structures regulate transcription elongation rates.Communications biology · 2024Article
- The structure and activities of the archaeal transcription termination factor Eta detail vulnerabilities of the transcription elongation complex.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Archaeal transcription.Transcription · 2020Review
- The Role of Archaeal Chromatin in Transcription.Journal of molecular biology · 2019Review
- TFS and Spt4/5 accelerate transcription through archaeal histone-based chromatin.Molecular microbiology · 2019Article
- Factor-dependent archaeal transcription termination.Proceedings of the National Academy of Sciences of the United States of America · 2017Article
- The GAN Exonuclease or the Flap Endonuclease Fen1 and RNase HII Are Necessary for Viability of Thermococcus kodakarensis.Journal of bacteriology · 2017Article
- Archaeal RNA polymerase arrests transcription at DNA lesions.Transcription · 2017Article
- Transcription Regulation in Archaea.Journal of bacteriology · 2016Review
- Analyses of in vivo interactions between transcription factors and the archaeal RNA polymerase.Methods (San Diego, Calif.) · 2015Article
Corrections and comments
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Authors and funding
2 authors.
Funding
Abstract
Transcription elongation by multisubunit RNA polymerases (RNAPs) is processive, but neither uniform nor continuous. Regulatory events during elongation include pausing, backtracking, arrest, and transcription termination, and it is critical to determine whether the absence of continued synthesis is transient or permanent. Here we describe mechanisms to generate large quantities of stable archaeal elongation complexes on a solid support to permit (1) single-round transcription, (2) walking of RNAP to any defined template position, and (3) discrimination of transcripts that are associated with RNAP from those that are released to solution. This methodology is based on untagged proteins transcribing biotin- and digoxigenin-labeled DNA templates in association with paramagnetic particles.
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