ArticleInternational journal of molecular sciences2015
Characterisation of two oxidosqualene cyclases responsible for triterpenoid biosynthesis in Ilex asprella.
Article in International journal of molecular sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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13 citing papers in PubMed, 36 citations in OpenAlex.
- Tissue-specific transcriptome analyses unveils candidate genes for flavonoid biosynthesis, regulation and transport in the medicinal plant Ilex asprella.Scientific reports · 2024Article
- Squalene hopene cyclases and oxido squalene cyclases: potential targets for regulating cyclisation reactions.Biotechnology letters · 2023Review
- Cytochrome P450 Monooxygenase/Cytochrome P450 Reductase Bi-Enzymatic System Isolated FromFrontiers in plant science · 2022Article
- Differential DNA methylation and gene expression during development of reproductive and vegetative organs in Ilex species.Journal of plant research · 2021Article
- The MYB transcription factor CiMYB42 regulates limonoids biosynthesis in citrus.BMC plant biology · 2020Article
- Identification of α-Amyrin 28-Carboxylase and Glycosyltransferase FromFrontiers in plant science · 2020Article
- Molecular Docking and Molecular Dynamics Studies on Selective Synthesis of α-Amyrin and β-Amyrin by Oxidosqualene Cyclases fromInternational journal of molecular sciences · 2019Article
- Complete plastome sequence ofMitochondrial DNA. Part B, Resources · 2019Article
- Antioxidant, α-Amylase and α-Glucosidase Inhibitory Activities and Potential Constituents ofMolecules (Basel, Switzerland) · 2019Article
- Reprogramming Tumor Associated Macrophage Phenotype by a Polysaccharide fromInternational journal of molecular sciences · 2018Article
- De novo sequencing, assembly and characterisation of Aloe vera transcriptome and analysis of expression profiles of genes related to saponin and anthraquinone metabolism.BMC genomics · 2018Article
- Cloning and characterization of squalene synthase and cycloartenol synthase fromActa pharmaceutica Sinica. B · 2017Article
- Transcriptomic Comparison Reveals Candidate Genes for Triterpenoid Biosynthesis in Two Closely RelatedFrontiers in plant science · 2017Article
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Authors and funding
10 authors at 1 institution in 1 country.
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No grant is acknowledged in the PubMed record.
Abstract
Ilex asprella, a plant widely used as a folk herbal drug in southern China, produces and stores a large amount of triterpenoid saponins, most of which are of the α-amyrin type. In this study, two oxidosqualene cyclase (OSC) cDNAs, IaAS1 and IaAS2, were cloned from the I. asprella root. Functional characterisation was performed by heterologous expression in the yeast Saccharomyces cerevisiae. Analysis of the resulting products by gas chromatography (GC) and gas chromatography-mass spectrometry (GC-MS) showed that both genes encode a mixed amyrin synthase, producing α-amyrin and β-amyrin at different ratios. IaAS1, which mainly produces α-amyrin, is the second triterpene synthase so far identified in which the level of α-amyrin produced is ≥80% of total amyrin production. By contrast, IaAS2 mainly synthesises β-amyrin, with a yield of 95%. Gene expression patterns of these two amyrin synthases in roots and leaves of I. asprella were found to be consistent with the content patterns of total saponins. Finally, phylogenetic analysis and multiple sequence alignment of the two amyrin synthases against several known OSCs from other plants were conducted to further elucidate their evolutionary relationship.
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