ArticleNature nanotechnology2015
Imaging and three-dimensional reconstruction of chemical groups inside a protein complex using atomic force microscopy.
Article in Nature nanotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
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Who cites it
13 citing papers in PubMed, 36 citations in OpenAlex.
- Beyond the Single Molecule: Multiplexed Methods in Force Spectroscopy.Annual review of biophysics · 2026Review
- Mapping Single-Molecule Protein Complexes in 3D with DNA Nanoswitch Calipers.Journal of the American Chemical Society · 2023Article
- Resolving Molecular Heterogeneity with Single-Molecule Centrifugation.Journal of the American Chemical Society · 2023Article
- Diamagnetically levitated nanopositioners with large-range and multiple degrees of freedom.Nature communications · 2022Article
- Biological physics by high-speed atomic force microscopy.Philosophical transactions. Series A, Mathematical, physical, and engineering sciences · 2020Review
- Calibration of T-shaped atomic force microscope cantilevers using the thermal noise method.The Review of scientific instruments · 2020Article
- Closed-loop atomic force microscopy-infrared spectroscopic imaging for nanoscale molecular characterization.Nature communications · 2020Article
- Next Generation Methods for Single-Molecule Force Spectroscopy on Polyproteins and Receptor-Ligand Complexes.Frontiers in molecular biosciences · 2020Review
- Allosterically Linked Binding Sites in Serotonin Transporter Revealed by Single Molecule Force Spectroscopy.Frontiers in molecular biosciences · 2020Review
- Vibration-Energy-Harvesting System: Transduction Mechanisms, Frequency Tuning Techniques, and Biomechanical Applications.Advanced materials technologies · 2019Article
- Imaging modes of atomic force microscopy for application in molecular and cell biology.Nature nanotechnology · 2017Review
- Structural biology: Peering inside protein complexes with AFM.Nature methods · 2015Article
- Atomic force microscopy as an advanced tool in neuroscience.Translational neuroscience · 2015Review
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2 authors at 1 institution in 1 country.
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Abstract
Scanning probe microscopes can be used to image and chemically characterize surfaces down to the atomic scale. However, the localized tip-sample interactions in scanning probe microscopes limit high-resolution images to the topmost atomic layer of surfaces, and characterizing the inner structures of materials and biomolecules is a challenge for such instruments. Here, we show that an atomic force microscope can be used to image and three-dimensionally reconstruct chemical groups inside a protein complex. We use short single-stranded DNAs as imaging labels that are linked to target regions inside a protein complex, and T-shaped atomic force microscope cantilevers functionalized with complementary probe DNAs allow the labels to be located with sequence specificity and subnanometre resolution. After measuring pairwise distances between labels, we reconstruct the three-dimensional structure formed by the target chemical groups within the protein complex using simple geometric calculations. Experiments with the biotin-streptavidin complex show that the predicted three-dimensional loci of the carboxylic acid groups of biotins are within 2 Å of their respective loci in the corresponding crystal structure, suggesting that scanning probe microscopes could complement existing structural biological techniques in solving structures that are difficult to study due to their size and complexity.
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