ArticlePloS one2015
Comparative analysis of super-shedder strains of Escherichia coli O157:H7 reveals distinctive genomic features and a strongly aggregative adherent phenotype on bovine rectoanal junction squamous epithelial cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 24 papers.
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Who cites it
24 citing papers in PubMed, 33 citations in OpenAlex.
- Investigating the adherence factors ofMicrobiology spectrum · 2024Article
- In Silico Analysis of Shiga Toxin-ProducingToxins · 2024Article
- Bovine Rectoanal Junction In Vitro Organ Culture Model System to Study Shiga Toxin-ProducingMicroorganisms · 2023Article
- Alteration of a Shiga toxin-encoding phage associated with a change in toxin production level and disease severity inMicrobial genomics · 2023Article
- Super Shedding in Enteric Pathogens: A Review.Microorganisms · 2022Review
- Article
- Mucosal IFNγ production and potential role in protection in Escherichia coli O157:H7 vaccinated and challenged cattle.Scientific reports · 2021Article
- Strain and host-cell dependent role of type-1 fimbriae in the adherence phenotype of super-shed Escherichia coli O157:H7.International journal of medical microbiology : IJMM · 2021Article
- A One Health Perspective for Defining and DecipheringComparative medicine · 2021Review
- Nonfimbrial Adhesin Mutants Reveal DivergentInternational journal of microbiology · 2021Article
- Genetic and Functional Analyses of Virulence Potential of anFrontiers in cellular and infection microbiology · 2020Article
- SupershedInternational journal of microbiology · 2020Article
- Circulation of Shiga Toxin-ProducingFrontiers in microbiology · 2020Article
- Bird feathers as potential sources of pathogenic microorganisms: a new look at old diseases.Antonie van Leeuwenhoek · 2018Review
- Conditional Function of Autoaggregative Protein Cah and CommonApplied and environmental microbiology · 2018Article
- Prevalence and Genomic Characterization of Escherichia coli O157:H7 in Cow-Calf Herds throughout California.Applied and environmental microbiology · 2017Article
- Escherichia coli, cattle and the propagation of disease.FEMS microbiology letters · 2017Review
- Curli Temper Adherence of Escherichia coli O157:H7 to Squamous Epithelial Cells from the Bovine Recto-Anal Junction in a Strain-Dependent Manner.Applied and environmental microbiology · 2017Article
- Article
- Whole Genome Sequencing for Genomics-Guided Investigations of Escherichia coli O157:H7 Outbreaks.Frontiers in microbiology · 2016Article
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Authors and funding
13 authors at 4 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Shiga toxin-producing Escherichia coli O157:H7 (O157) are significant foodborne pathogens and pose a serious threat to public health worldwide. The major reservoirs of O157 are asymptomatic cattle which harbor the organism in the terminal recto-anal junction (RAJ). Some colonized animals, referred to as "super-shedders" (SS), are known to shed O157 in exceptionally large numbers (>104 CFU/g of feces). Recent studies suggest that SS cattle play a major role in the prevalence and transmission of O157, but little is known about the molecular mechanisms associated with super-shedding. Whole genome sequence analysis of an SS O157 strain (SS17) revealed a genome of 5,523,849 bp chromosome with 5,430 open reading frames and two plasmids, pO157 and pSS17, of 94,645 bp and 37,446 bp, respectively. Comparative analyses showed that SS17 is clustered with spinach-associated O157 outbreak strains, and belongs to the lineage I/II, clade 8, D group, and genotype 1, a subgroup of O157 with predicted hyper-virulence. A large number of non-synonymous SNPs and other polymorphisms were identified in SS17 as compared with other O157 strains (EC4115, EDL933, Sakai, TW14359), including in key adherence- and virulence-related loci. Phenotypic analyses revealed a distinctive and strongly adherent aggregative phenotype of SS17 on bovine RAJ stratified squamous epithelial (RSE) cells that was conserved amongst other SS isolates. Molecular genetic and functional analyses of defined mutants of SS17 suggested that the strongly adherent aggregative phenotype amongst SS isolates is LEE-independent, and likely results from a novel mechanism. Taken together, our study provides a rational framework for investigating the molecular mechanisms associated with SS, and strong evidence that SS O157 isolates have distinctive features and use a LEE-independent mechanism for hyper-adherence to bovine rectal epithelial cells.
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