Cloning and expression of visfatin and screening of oligopeptides binding with visfatin.

Xin Yu Liu, Lin Ge, De Min Yu

Open access · greenAbstract read

In one paragraph

Article in International journal of clinical and experimental medicine, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 1 paper.

0numbers the graph read from it

0cells of the map it votes in

1citing papers in PubMed

0.7field-weighted citation impact, top 29% of its field

1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

1 citing paper in PubMed, 5 citations in OpenAlex.

Nicotinamide phosphoribosyltransferase purification using SUMO expression system.Analytical biochemistry · 2020
Article

4 · The record

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PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 1 country.

Xin Yu LiuMinistry of Health Key Laboratory of Hormone and Development, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China ; Department of Diabetic Gastroenterology, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China.

Lin GeDepartment of Biochemistry and Molecular Biology, Basic Medical College, Tianjin Medical University Tianjin 300070, P. R. China ; Tianjin Key Laboratory of Cellular and Molecular Immunology Tianjin 300070, P. R. China ; Key Laboratory of Educational Ministry of China Tianjin 300070, P. R. China.

De Min YuMinistry of Health Key Laboratory of Hormone and Development, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China ; Department of Diabetic Gastroenterology, Metabolic Diseases Hospital, Tianjin Medical University Tianjin 300070, P. R. China.

Tianjin Infectious Diseases Hospital · CNTianjin Medical University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

purposeIn the present study, we constructed an efficient expression system for visfatin and identified the peptide sequences which binding to visfatin. METHODS AND

resultsThe fusion protein was identified by SDS-PAGE and Western blot. The yield of visfatin was 300 mg/L culture medium with optimal conditions. The recombinant visfatin binds with insulin receptor with a dose-dependent effect. All of the 24 sequence identified by ELISA were able to bind to visfatin specifically. Among the 24 DNA sequences, there were 8 clones of AAKTPTE, 4 clones of ATTVPAS and 4 clones of MSLQQEH.

conclusionThe sequence of AA(X)TPT(X) was the most frequently existed sequence in all of sequences analyzed, which suggests that AA(X)TPT(X) is likely to be the essential motif in peptides which visfatin could bind with.

Indexed as

adipokinefusion expressiongene engineeringphage displayVisfatin

Identifiers

PMID25663979

PMCPMC4307426

OpenAlexW2194940942

What OpenQuestion holds

Textmetadata

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.