Evidence map›Paper›PMID 25663850›Full record

ArticleAnalytical methods : advancing methods and applications2015

Enhancement of Colorimetric Response of Enzymatic Reactions by Thermally Evaporated Plasmonic Thin Films: Application to Glial Fibrillary Acidic Protein.

Biebele Abel, Tabassum S Kabir, Babatunde Odukoya, Muzaffer Mohammed, Kadir Aslan

Abstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Analytical methods : advancing methods and applications, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 2 papers.

0numbers the graph read from it
0cells of the map it votes in
2citing papers in PubMed
–field-weighted citation impact
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

2 citing papers in PubMed.

  1. Metal oxide surfaces for enhanced colorimetric response in bioassays.Colloids and surfaces. B, Biointerfaces · 2017
    Article
  2. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

5 authors.

Biebele AbelMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.
Tabassum S KabirMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.
Babatunde OdukoyaMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.
Muzaffer MohammedMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.
Kadir AslanMorgan State University, Department of Chemistry, 1700 East Cold Spring Lane, Baltimore, MD 21251 USA.

Funding

Ultra Fast and Sensitive Fluorescence-Based Clinical AssaysK25EB007565 · NIBIB · UNIVERSITY OF MD BIOTECHNOLOGY INSTITUTE · PI ASLAN, KADIR · 2008 to 2011
$607k
NIBIB NIH HHS K25 EB007565
6 · The paper itself

Abstract

We report the enhancement of the colorimetric response of horseradish peroxidase (HRP) and alkaline phosphatase (AP) in bioassays by thermally evaporated silver, gold, copper and nickel thin films. In this regard, a model bioassay based on biotin-avidin interactions was employed. Biotin groups and enzymes were introduced to all surfaces using a biotinylated linker molecule and avidin, respectively. The colorimetric response of HRP in the model bioassay carried out on the plasmonic thin films were up to 4.4-fold larger as compared to control samples (i.e., no plasmonic thin films), where the largest enhancement of colorimetric response was observed on silver thin films. The colorimetric response of AP on plasmonic thin films was found to be similar to those observed on control samples, which was attributed to the loss of enzymes from the surface during the bioassay steps. The extent of enzymes immobilized on to plasmonic thin films was found to affect the colorimetric response of the model bioassay. These findings allowed us to demonstrate the use of silver thin films for the detection of glial fibrillary acidic protein (GFAP), where the colorimetric response of the standard bioassays for GFAP was enhanced up to 67% as compared to bioassays on glass slides.

Indexed as

alkaline phosphataseamplification of colorimetric responsebiotin-poly(ethylene-glycol)aminecopper thin filmsglial fibrillary acidic proteingold thin filmshorse radish peroxidasenickel thin filmsPlasmonic thin filmssilver thin films

Identifiers

PMID25663850
PMCPMC4318255

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.