ArticleStem cells international2015
Peripheral blood derived mononuclear cells enhance the migration and chondrogenic differentiation of multipotent mesenchymal stromal cells.
Article in Stem cells international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 13 papers.
What it found
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- High-Dose Neutrophil-Depleted Platelet-Rich Plasma Therapy for Knee Osteoarthritis: A Retrospective Study.Journal of clinical medicine · 2024Article
- PRISMA-Compliant Meta-Analysis of Randomized Controlled Trials on Osteoarthritis of Knee Managed with Allogeneic vs Autologous MSCs: Efficacy and Safety Analysis.Indian journal of orthopaedics · 2022Review
- Autologous Immune Cell-Based Regenerative Therapies to Treat Vasculogenic Erectile Dysfunction: Is the Immuno-Centric Revolution Ready for the Prime Time?Biomedicines · 2022Review
- Using apheresis-derived cells to augment microdrilling in the treatment of chondral defects in an ovine model.Journal of orthopaedic research : official publication of the Orthopaedic Research Society · 2021Article
- Chemokine-Induced PBMC and Subsequent MSC Migration Toward Decellularized Heart Valve Tissue.Cardiovascular engineering and technology · 2021Article
- The Use of Peripheral Blood-Derived Stem Cells for Cartilage Repair and RegenerationFrontiers in pharmacology · 2020Review
- Gene expression and functional comparison between multipotential stromal cells from lateral and medial condyles of knee osteoarthritis patients.Scientific reports · 2019Article
- Comparative advantages of infrapatellar fat pad: an emerging stem cell source for regenerative medicine.Rheumatology (Oxford, England) · 2018Review
- miR‑330‑5p inhibits H2O2‑induced adipogenic differentiation of MSCs by regulating RXRγ.International journal of molecular medicine · 2018Article
- Peripheral mononuclear blood cell apheresis in a preclinical ovine model.BMC veterinary research · 2018Article
- Article
- Peripheral blood derived mononuclear cells enhance osteoarthritic human chondrocyte migration.Arthritis research & therapy · 2015Article
- Peripheral Blood Mononuclear Cells Enhance Cartilage Repair in in vivo Osteochondral Defect Model.PloS one · 2015Article
Corrections and comments
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
A major challenge in cartilage repair is the lack of chondrogenic cells migrating from healthy tissue into damaged areas and strategies to promote this should be developed. The aim of this study was to evaluate the effect of peripheral blood derived mononuclear cell (PBMC) stimulation on mesenchymal stromal cells (MSCs) derived from the infrapatellar fat pad of human OA knee. Cell migration was measured using an xCELLigence electronic migration chamber system in combination with scratch assays. Gene expression was quantified with stem cell PCR arrays and validated using quantitative real-time PCR (rtPCR). In both migration assays PBMCs increased MSC migration by comparison to control. In scratch assay the wound closure was 55% higher after 3 hours in the PBMC stimulated test group (P = 0.002), migration rate was 9 times faster (P = 0.008), and total MSC migration was 25 times higher after 24 hours (P = 0.014). Analysis of MSCs by PCR array demonstrated that PBMCs induced the upregulation of genes associated with chondrogenic differentiation over 15-fold. In conclusion, PBMCs increase both MSC migration and differentiation suggesting that they are an ideal candidate for inclusion in regenerative medicine therapies aimed at cartilage repair.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.