Evidence map›Paper›PMID 25663822›Full record

ArticleCancer cell international2014

A novel GFP nude rat model to investigate tumor-stroma interactions.

Ning Yang, Bin Huang, Oleg Tsinkalovsky, Narve Brekkå, Huaiyang Zhu, Lina Leiss, Per Øyvind Enger, Xingang Li, Jian Wang

Open access · goldAbstract read
PubMed Full text at PMC Publisher
In one paragraph

Article in Cancer cell international, 2014. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 3 papers.

0numbers the graph read from it
0cells of the map it votes in
3citing papers in PubMed
0.3field-weighted citation impact, top 32% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

3 citing papers in PubMed, 4 citations in OpenAlex.

  1. Review
  2. Review
  3. Article
4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

9 authors at 3 institutions in 2 countries.

Ning YangDepartment of Neurosurgery, Qilu Hospital of Shandong University, Jinan, China ; Department of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; Brain Science Research Institute, Shandong University, Jinan, China.
Bin HuangDepartment of Neurosurgery, Qilu Hospital of Shandong University, Jinan, China ; Brain Science Research Institute, Shandong University, Jinan, China.
Oleg TsinkalovskyDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway.
Narve BrekkåDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway.
Huaiyang ZhuDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway.
Lina LeissDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; Neuro Clinic, Haukeland University Hospital, Bergen, Norway.
Per Øyvind EngerDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; Department of Neurosurgery, Haukeland University Hospital, Bergen, Norway.
Xingang LiDepartment of Neurosurgery, Qilu Hospital of Shandong University, Jinan, China ; Brain Science Research Institute, Shandong University, Jinan, China.
Jian WangDepartment of Biomedicine, University of Bergen, N-5009 Bergen, Norway ; Brain Science Research Institute, Shandong University, Jinan, China.
University of Bergen · NOQilu Hospital of Shandong University · CNShandong University · CN

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

backgroudA key strategy for the study of the tumor microenvironment is to implant human tumor cells in an immunodeficient rodent strain ubiquitously expressing a fluorescent marker. Here, a novel nude rat expressing a green fluorescent protein (GFP) transgene was established and engrafted with primary human tumor tissue in order to separate tumor from stromal cell populations for subsequent molecular analysis.

methodsSD-TG (GFP) 2BalRrrc transgenic rats were crossed with HsdHan™: rnu/rnu Rowett nude rats to develop a GFP expressing immunocompromised rat. PCR and flow cytometry were used to follow the GFP genotype and phenotype in newborns. After three to four generations, animals were implanted with 4 T1 dsRed murine breast cancer cells or primary human glioblastoma (GBM) biopsies to generate xenografts for subsequent separation by fluorescence-activated cell sorting (FACS).

resultsFluorecence microscopy and reverse transcription-PCR demonstrated that GFP, under the control of the human ubiquitin C promoter, was stably maintained and expressed in diverse organs over several generations. Immunophenotyping of blood samples by flow cytometry confirmed that the immunodeficient features of the parental rat strain, HsdHan™: rnu/rnu, were retained in the GFP nude rat. Both the murine cell line and human GBM biopsies engrafted, and stromal cell populations were isolated from dissociated xenografts by FACS to > 95% purity.

conclusionsA GFP transgene was stably introduced into a nude rat background in which human and murine cancer cells successfully engrafted. This animal strain provides a novel in vivo system for detailed cellular and molecular characterization of tumor-stroma interactions.

Indexed as

Breast cancerGlioblastomaTumor biologyTumor-stroma interactionXenograft

Identifiers

PMID25663822
PMCPMC4319225
OpenAlexW2112915268

What OpenQuestion holds

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LicenceCC BY
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Registered trials

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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.