ArticlePlanta2015
Mining NGS transcriptomes for miRNAs and dissecting their role in regulating growth, development, and secondary metabolites production in different organs of a medicinal herb, Picrorhiza kurroa.
Article in Planta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 31 papers.
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Who cites it
31 citing papers in PubMed, 77 citations in OpenAlex.
- Direct shoot regeneration and gene expression profiling for optimized picroside-I biosynthesis in Picrorhiza Kurroa.Scientific reports · 2025Article
- Function of floral fragrance-related microRNAs and their targets in Hedychium coronarium.BMC genomics · 2025Article
- Analysis of the miRNA Transcriptome inInternational journal of molecular sciences · 2025Article
- Relationship between Protein, MicroRNA Expression in Extracellular Vesicles and Rice Seed Vigor.International journal of molecular sciences · 2024Article
- Application and development of CRISPR technology in the secondary metabolic pathway of the active ingredients of phytopharmaceuticals.Frontiers in plant science · 2024Review
- Integrated mRNA and small RNA sequencing reveals post-transcriptional regulation of the sesquiterpene pathway in3 Biotech · 2023Article
- Multilayered regulation of secondary metabolism in medicinal plants.Molecular horticulture · 2023Review
- A Brief Review of Plant Cell Transfection, Gene Transcript Expression, and Genotypic Integration for Enhancing Compound Production.Methods in molecular biology (Clifton, N.J.) · 2023Review
- Identification of miRNAs and their targets in twoFrontiers in plant science · 2023Article
- MicroRNAs in Medicinal Plants.International journal of molecular sciences · 2022Review
- An insight into microRNA biogenesis and its regulatory role in plant secondary metabolism.Plant cell reports · 2022Review
- Integrative Analysis of miRNAs and Their Targets Involved in Ray Floret Growth inInternational journal of molecular sciences · 2022Article
- Integrated mRNA and miRNA Transcriptome Analysis Suggests a Regulatory Network for UV-B-Controlled Terpenoid Synthesis in Fragrant Woodfern (International journal of molecular sciences · 2022Article
- Biosynthesis of Secondary Metabolites Based on the Regulation of MicroRNAs.BioMed research international · 2022Review
- Identification of microRNAs from Medicinal PlantPlants (Basel, Switzerland) · 2021Article
- Genome-Wide Characterization of Salt-Responsive miRNAs, circRNAs and Associated ceRNA Networks in Tomatoes.International journal of molecular sciences · 2021Article
- Transcriptome repository of North-Western Himalayan endangered medicinal herbs: a paramount approach illuminating molecular perspective of phytoactive molecules and secondary metabolism.Molecular genetics and genomics : MGG · 2021Review
- Complexity of gene paralogues resolved in biosynthetic pathway of hepatoprotective iridoid glycosides in a medicinal herb, Picrorhiza kurroa through differential NGS transcriptomes.Molecular genetics and genomics : MGG · 2021Article
- Recent Advances in Heterologous Synthesis Paving Way for Future Green-Modular Bioindustries: A Review With Special Reference to Isoflavonoids.Frontiers in bioengineering and biotechnology · 2021Review
- Integrated Four Comparative-Omics Reveals the Mechanism of the Terpenoid Biosynthesis in Two Different OverwinteringFrontiers in plant science · 2021Article
Corrections and comments
- Erratum issued
Authors and funding
6 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
MAIN
conclusionThis study is the first endeavor on mining of miRNAs and analyzing their involvement in development and secondary metabolism of an endangered medicinal herb Picrorhiza kurroa (P. kurroa ). miRNAs are ubiquitous non-coding RNA species that target complementary sequences of mRNA and result in either translational repression or target degradation in eukaryotes. The role of miRNAs has not been investigated in P. kurroa which is a medicinal herb of industrial value due to the presence of secondary metabolites, picroside-I and picroside-II. Computational identification of miRNAs was done in 6 transcriptomes of P. kurroa generated from root, shoot, and stolon organs varying for growth, development, and culture conditions. All available plant miRNA entries were retrieved from miRBase and used as backend datasets to computationally identify conserved miRNAs in transcriptome data sets. Total 18 conserved miRNAs were detected in P. kurroa followed by target prediction and functional annotation which suggested their possible role in controlling various biological processes. Validation of miRNA and expression analysis by qRT-PCR and 5' RACE revealed that miRNA-4995 has a regulatory role in terpenoid biosynthesis ultimately affecting the production of picroside-I. miR-5532 and miR-5368 had negligible expression in field-grown samples as compared to in vitro-cultured samples suggesting their role in regulating P. kurroa growth in culture conditions. The study has thus identified novel functions for existing miRNAs which can be further validated for their potential regulatory role.
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