ArticleJournal of industrial microbiology & biotechnology2015
Engineered Serratia marcescens for efficient (3R)-acetoin and (2R,3R)-2,3-butanediol production.
Article in Journal of industrial microbiology & biotechnology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 18 papers.
What it found
Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.
The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
The trial behind it
Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.
Who cites it
18 citing papers in PubMed, 47 citations in OpenAlex.
- Exploring ethyl methane sulphonate induced, L-cysteine HCl resistant Bacillus subtilis putative mutant and its immobilization for enhanced acetoin synthesis.Archives of microbiology · 2026Article
- Mechanism of microbial production of acetoin and 2,3-butanediol optical isomers and substrate specificity of butanediol dehydrogenase.Microbial cell factories · 2023Review
- Production of highly pure R,R-2,3-butanediol for biological plant growth promoting agent using carbon feeding control of Paenibacillus polymyxa MDBDO.Microbial cell factories · 2023Article
- Metabolic Engineering ofMicroorganisms · 2023Article
- PtrA regulates prodigiosin synthesis and biological functions inFrontiers in microbiology · 2023Article
- C4 Bacterial Volatiles Improve Plant Health.Pathogens (Basel, Switzerland) · 2021Review
- Engineering central pathways for industrial-level (3R)-acetoin biosynthesis in Corynebacterium glutamicum.Microbial cell factories · 2020Article
- The current strategies and parameters for the enhanced microbial production of 2,3-butanediol.Biotechnology reports (Amsterdam, Netherlands) · 2020Article
- Metabolic characterization of the chitinolytic bacterium Serratia marcescens using a genome-scale metabolic model.BMC bioinformatics · 2019Article
- Structure-guided design of Serratia marcescens short-chain dehydrogenase/reductase for stereoselective synthesis of (R)-phenylephrine.Scientific reports · 2018Article
- Production of (2R, 3R)-2,3-butanediol using engineeredBiotechnology for biofuels · 2018Article
- Electrode-assisted acetoin production in a metabolically engineeredBiotechnology for biofuels · 2017Article
- Efficient production of acetoin in Saccharomyces cerevisiae by disruption of 2,3-butanediol dehydrogenase and expression of NADH oxidase.Scientific reports · 2016Article
- Sweet scents from good bacteria: Case studies on bacterial volatile compounds for plant growth and immunity.Plant molecular biology · 2016Review
- Mechanism of 2,3-butanediol stereoisomers formation in a newly isolated Serratia sp. T241.Scientific reports · 2016Article
- Metabolic engineering ofBioresources and bioprocessing · 2016Article
- Impact of a Bacterial Volatile 2,3-Butanediol on Bacillus subtilis Rhizosphere Robustness.Frontiers in microbiology · 2016Article
- Metabolic engineering of Escherichia coli for production of (2S,3S)-butane-2,3-diol from glucose.Biotechnology for biofuels · 2015Article
Corrections and comments
- Erratum issued
- Erratum issued
Authors and funding
7 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
(3R)-Acetoin and (2R,3R)-2,3-butanediol are important pharmaceutical intermediates. However, until now, the quantity of natural microorganisms with the ability to produce single configuration of optically pure (3R)-acetoin and (2R,3R)-2,3-butanediol is rare. In this study, a meso-2,3-butanediol dehydrogenase encoded by the slaC gene from Serratia marcescens MG1 was identified for meso-2,3-butanediol and (2S,3S)-2,3-butanediol biosynthesis. Inactivation of the slaC gene could significantly decrease meso-2,3-butanediol and (2S,3S)-2,3-butanediol and result in a large quantity of (3R)-acetoin accumulation. Furthermore, a (2R,3R)-2,3-butanediol dehydrogenase encoded by the bdhA gene from Bacillus subtilis 168 was introduced into the slaC mutant strain of Serratia marcescens MG1. Excess (2R,3R)-2,3-butanediol dehydrogenase could accelerate the reaction from (3R)-acetoin to (2R,3R)-2,3-butanediol and lead to (2R,3R)-2,3-butanediol accumulation. In fed-batch fermentation, the excess (2R,3R)-2,3-butanediol dehydrogenase expression strain could produce 89.81 g/l (2R,3R)-2,3-butanediol with a productivity of 1.91 g/l/h at 48 h. These results provided potential applications for (3R)-acetoin and (2R,3R)-2,3-butanediol production.
Indexed as
Identifiers
What OpenQuestion holds
Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.