ArticleJournal of plant research2015
AgarTrap-mediated genetic transformation using intact gemmae/gemmalings of the liverwort Marchantia polymorpha L.
Article in Journal of plant research, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 27 papers.
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27 citing papers in PubMed, 51 citations in OpenAlex.
- A key role for S-nitrosylation in immune regulation and development in the liverwort Marchantia polymorpha.Journal of experimental botany · 2026Article
- The MpCAFA gene encodes a ciliary protein required for spermatozoid motility in the liverwort Marchantia polymorpha.Journal of plant research · 2025Article
- Optimization of selection agent concentrations and expanding G418 utility for gentamicin resistance in Marchantia polymorpha.Scientific reports · 2025Article
- A Simple and Scalable Chopped-Thallus Transformation Method forPlants (Basel, Switzerland) · 2025Article
- Growth control of Marchantia polymorpha gemmae using nonthermal plasma irradiation.Scientific reports · 2024Article
- Selection of a histidine auxotrophicPlant biotechnology (Tokyo, Japan) · 2022Article
- The localization of phototropin to the plasma membrane defines a cold-sensing compartment inPNAS nexus · 2022Article
- Image-Based Analysis Revealing the Molecular Mechanism of Peroxisome Dynamics in Plants.Frontiers in cell and developmental biology · 2022Review
- Transformation ofInternational journal of molecular sciences · 2020Article
- Plant science's next top models.Annals of botany · 2020Review
- Article
- The cold-induced switch in direction of chloroplast relocation occurs independently of changes in endogenous phototropin levels.PloS one · 2020Article
- A gain-of-function T-DNA insertion mutant ofPlant biotechnology (Tokyo, Japan) · 2019Article
- Relationship between relocation of phototropin to the chloroplast periphery and the initiation of chloroplast movement inPlant direct · 2019Article
- Highly efficient G-AgarTrap-mediated transformation of thePlant biotechnology (Tokyo, Japan) · 2018Article
- Improved G-AgarTrap: A highly efficient transformation method for intact gemmalings of the liverwort Marchantia polymorpha.Scientific reports · 2018Article
- AgarTrap Protocols on your Benchtop: Simple Methods forPlant biotechnology (Tokyo, Japan) · 2018Article
- Efficient CRISPR/Cas9-based genome editing and its application to conditional genetic analysis in Marchantia polymorpha.PloS one · 2018Article
- Article
- Chloroplast aggregation during the cold-positioning response in the liverwort Marchantia polymorpha.Journal of plant research · 2017Article
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2 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The dioecious liverwort, Marchantia polymorpha L., is an emerging model plant. Various molecular biological techniques have been optimized for M. polymorpha for the past several years, and recently we reported a simplified Agrobacterium-mediated transformation method using sporelings (immature thalli from spores) of M. polymorpha. This method, termed AgarTrap (Agar-utilized Transformation with Pouring Solutions), completed by exchanging appropriate solutions on a single Petri dish to produce a sufficient number of independent transgenic sporelings. However, because spores are produced by crosses between males and females, the genetic backgrounds of resulting transgenic sporelings are not uniform. To easily produce transgenic liverworts with a uniform genetic background using AgarTrap, we developed an AgarTrap-mediated transformation method using intact gemmae/gemmalings produced by asexual reproduction. Using AgarTrap with male and female gemmae/gemmalings produced a sufficient number of independent transgenic gemmalings with uniform genetic backgrounds. The optimized transformation efficiencies were approximately 30 and 50 % in males and females, respectively. As with AgarTrap using sporelings, AgarTrap using intact gemmae/gemmalings will be useful in promoting studies of the molecular biology of M. polymorpha.
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