ArticleChromosoma2015
Dynamics of hnRNPs and omega speckles in normal and heat shocked live cell nuclei of Drosophila melanogaster.
Article in Chromosoma, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
22 citing papers in PubMed.
- Oligo(dT) Fluorescence In Situ Hybridization to Visualize the Poly(A) mRNAs in the Internal Tissues ofBio-protocol · 2026Article
- An snRNA-seq aging clock for the fruit fly head sheds light on sex-biased aging.Scientific reports · 2026Article
- Novel roles for RNA binding proteins squid, hephaesteus, and Hrb27C in Drosophila oogenesis.Developmental dynamics : an official publication of the American Association of Anatomists · 2023Article
- Recent Advances and Future Potential of Long Non-Coding RNAs in Insects.International journal of molecular sciences · 2023Review
- Hsrω and Other lncRNAs in Neuronal Functions and Disorders inLife (Basel, Switzerland) · 2022Review
- Review
- Review
- Keeping up with the condensates: The retention, gain, and loss of nuclear membrane-less organelles.Frontiers in molecular biosciences · 2022Review
- Long Non-Coding RNAs in Insects.Animals : an open access journal from MDPI · 2021Review
- Amyotrophic Lateral Sclerosis Genes inInternational journal of molecular sciences · 2021Review
- Emerging Roles of Repetitive and Repeat-Containing RNA in Nuclear and Chromatin Organization and Gene Expression.Frontiers in cell and developmental biology · 2021Review
- Short Tandem Repeat-Enriched Architectural RNAs in Nuclear Bodies: Functions and Associated Diseases.Non-coding RNA · 2020Review
- Conservation of gene architecture and domains amidst sequence divergence in theJournal of genetics · 2020Article
- Review
- Over-expression of Hsp83 in grossly depletedJournal of biosciences · 2019Article
- Article
- Bantam regulates the axonal geometry of Drosophila larval brain by modulating actin regulator enabled.Invertebrate neuroscience : IN · 2018Article
- Loss of ISWI Function in Drosophila Nuclear Bodies Drives Cytoplasmic Redistribution of Drosophila TDP-43.International journal of molecular sciences · 2018Article
- FUS toxicity is rescued by the modulation of lncRNA hsrω expression in Drosophila melanogaster.Scientific reports · 2017Article
- The hnRNP A1 homolog Hrb87F/Hrp36 is important for telomere maintenance in Drosophila melanogaster.Chromosoma · 2016Article
Corrections and comments
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Authors and funding
2 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The nucleus limited long-noncoding hsrω-n transcripts, hnRNPs, and some other RNA processing proteins organize nucleoplasmic omega speckles in Drosophila. Unlike other nuclear speckles, omega speckles rapidly disappear following cell stress, while hnRNPs and other associated proteins move away from chromosome sites, nucleoplasm, and the disappearing speckles to get uniquely sequestered at hsrω locus. Omega speckles reappear and hnRNPs get redistributed to normal locations during recovery from stress. With a view to understand the dynamics of omega speckles and their associated proteins, we used live imaging of GFP tagged hnRNPs (Hrb87F, Hrb98DE, or Squid) in unstressed and stressed Drosophila cells. Omega speckles display size-dependent mobility in nucleoplasmic domains with significant colocalization with nuclear matrix Tpr/Megator and SAFB proteins, which also accumulate at hsrω gene site after stress. Instead of moving towards the nuclear periphery located hsrω locus following heat shock or colchicine treatment, omega speckles rapidly disappear within nucleoplasm while chromosomal and nucleoplasmic hnRNPs move, stochastically or, more likely, by nuclear matrix-mediated transport to hsrω locus in non-particulate form. Continuing transcription of hsrω during cell stress is essential for sequestering incoming hnRNPs at the site. While recovering from stress, the sequestered hnRNPs are released as omega speckles in ISWI-dependent manner. Photobleaching studies reveal hnRNPs to freely move between nucleoplasm, omega speckles, chromosome regions, and hsrω gene site although their residence periods at chromosomes and hsrω locus are longer. A model for regulation of exchange of hnRNPs between nuclear compartments by hsrω-n transcripts is presented.
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Registered trials
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