ArticleMolecular neurobiology2016
Normalization of Reverse Transcription Quantitative PCR Data During Ageing in Distinct Cerebral Structures.
Article in Molecular neurobiology, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers, 1 of them a synthesis that pooled it.
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17 citing papers in PubMed, 1 synthesis or guideline pooled it.
- A systematic review on the selection of reference genes for gene expression studies in rodents: are the classics the best choice?Molecular biology reports · 2024Pooled it
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- Age-associated increases in inter-individual gene expression variability across human tissues.bioRxiv : the preprint server for biology · 2026Article
- Genome-wide DNA methylation analysis of hippocampal tissue in a murine model of attention deficit-hyperactivity disorder.PloS one · 2025Article
- A Purine Derivative Containing an Organoselenium Group Protects Against Memory Impairment, Sensitivity to Nociception, Oxidative Damage, and Neuroinflammation in a Mouse Model of Alzheimer's Disease.Molecular neurobiology · 2023Article
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- Bioinformatic Study of Transcriptome Changes in the Mice Lumbar Spinal Cord After the 30-Day Spaceflight and Subsequent 7-Day Readaptation on Earth: New Insights Into Molecular Mechanisms of the Hypogravity Motor Syndrome.Frontiers in pharmacology · 2019Article
- Reduction of HIP2 expression causes motor function impairment and increased vulnerability to dopaminergic degeneration in Parkinson's disease models.Cell death & disease · 2018Article
- Extracellular Matrix Modulation Is Driven by Experience-Dependent Plasticity During Stroke Recovery.Molecular neurobiology · 2018Article
- miRNA Signature of Hepatocellular Carcinoma Vascularization: How the Controls Can Influence the Signature.Digestive diseases and sciences · 2017Article
- Activation of cell surface GRP78 decreases endoplasmic reticulum stress and neuronal death.Cell death and differentiation · 2017Article
- Expression of acid-sensing ion channels and selection of reference genes in mouse and naked mole rat.Molecular brain · 2016Article
- Evaluation of candidate reference genes for RT-qPCR studies in three metabolism related tissues of mice after caloric restriction.Scientific reports · 2016Article
- Simultaneous Detection of Herpes Simplex Virus Type 1 Latent and Lytic Transcripts in Brain Tissue.ASN neuroArticle
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Reverse transcription quantitative-polymerase chain reaction (RT-qPCR) has become a routine method in many laboratories. Normalization of data from experimental conditions is critical for data processing and is usually achieved by the use of a single reference gene. Nevertheless, as pointed by the Minimum Information for Publication of Quantitative Real-Time PCR Experiments (MIQE) guidelines, several reference genes should be used for reliable normalization. Ageing is a physiological process that results in a decline of many expressed genes. Reliable normalization of RT-qPCR data becomes crucial when studying ageing. Here, we propose a RT-qPCR study from four mouse brain regions (cortex, hippocampus, striatum and cerebellum) at different ages (from 8 weeks to 22 months) in which we studied the expression of nine commonly used reference genes. With the use of two different algorithms, we found that all brain structures need at least two genes for a good normalization step. We propose specific pairs of gene for efficient data normalization in the four brain regions studied. These results underline the importance of reliable reference genes for specific brain regions in ageing.
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