ArticleExperimental parasitology
Comparison of diagnostic techniques for the detection of Cryptosporidium oocysts in animal samples.
Article in Experimental parasitology. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 22 papers.
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Who cites it
22 citing papers in PubMed, 50 citations in OpenAlex.
- Molecular survey of Cryptosporidium spp. in ornamental birds and their sellers: implications for One Health.BMC veterinary research · 2026Article
- Prevalence of cryptosporidiosis and other enteric pathogens in calves in France: effects of rotavirus, coronavirus and E. coli vaccination and transmission routes.Veterinary research · 2026Article
- Advances in diagnosis of diseases causing diarrhea in newborn calves.Veterinary research communications · 2025Review
- Prevalence and risk factors ofVeterinarni medicina · 2025Article
- Coproantigen detection and molecular identification of Cryptosporidium species among newborn and adult farm animals.AMB Express · 2025Article
- Comparison of four diagnostic techniques forQatar medical journal · 2025Article
- Intestinal Parasitic Infections in Ghana: A Review of Infections and the Risk of Zoonotic Transmission.BioMed research international · 2025Review
- Rapid detection ofHeliyon · 2023Article
- Diagnosis and control of cryptosporidiosis in farm animals.Journal of parasitic diseases : official organ of the Indian Society for Parasitology · 2022Review
- Performance of diagnostic assays used to detect Cryptosporidium oocysts in faecal samples of cattle in Kuwait and genotyping of Cryptosporidium species.BMC veterinary research · 2022Article
- Apicomplexans in Goat: Prevalence ofAnimals : an open access journal from MDPI · 2022Article
- Development and Preliminary Evaluation of a Nanoparticle-Assisted PCR Assay for the Detection ofAnimals : an open access journal from MDPI · 2022Article
- Population genetics of Cryptosporidium parvum subtypes in cattle in Poland: the geographical change of strain prevalence and circulation over time.BMC veterinary research · 2022Article
- A comparison of assays for the detection ofJournal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc · 2022Article
- Performance of a rapid diagnostic test for the detection of Cryptosporidium spp. in African children admitted to hospital with diarrhea.PLoS neglected tropical diseases · 2020Article
- New insights into the detection and molecular characterization of Cryptosporidium with emphasis in Brazilian studies: a review.Revista do Instituto de Medicina Tropical de Sao Paulo · 2019Review
- Cryptosporidium-associated diarrhoea in neonatal calves in Algeria.Veterinary parasitology, regional studies and reports · 2018Article
- Genetic uniqueness of Cryptosporidium parvum from dairy calves in Colombia.Parasitology research · 2018Article
- Cryptosporidium infection in children with cancer undergoing chemotherapy: how important is the prevention of opportunistic parasitic infections in patients with malignancies?Parasitology research · 2017Article
- Multilocus typing of Cryptosporidium spp. in young calves with diarrhea in Korea.Veterinary parasitology · 2016Article
Corrections and comments
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
Abstract
While a large number of laboratory methods for the detection of Cryptosporidium oocysts in faecal samples are now available, their efficacy for identifying asymptomatic cases of cryptosporidiosis is poorly understood. This study was carried out to determine a reliable screening test for epidemiological studies in livestock. In addition, three molecular tests were compared to identify Cryptosporidium species responsible for the infection in cattle, sheep and horses. A variety of diagnostic tests including microscopic (Kinyoun's staining), immunological (Direct Fluorescence Antibody tests or DFAT), enzyme-linked immunosorbent assay (ELISA), and molecular methods (nested PCR) were compared to assess their ability to detect Cryptosporidium in cattle, horse and sheep faecal samples. The results indicate that the sensitivity and specificity of each test is highly dependent on the input samples; while Kinyoun's and DFAT proved to be reliable screening tools for cattle samples, DFAT and PCR analysis (targeted at the 18S rRNA gene fragment) were more sensitive for screening sheep and horse samples. Finally different PCR primer sets targetedat the same region resulted in the preferential amplification of certain Cryptosporidium species when multiple species were present in the sample. Therefore, for identification of Cryptosporidium spp. in the event of asymptomatic cryptosporidiosis, the combination of different 18S rRNA nested PCR primer sets is recommended for further epidemiological applications and also tracking the sources of infection.
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Registered trials
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