ArticleScientific reports2015
Enhanced targeted integration mediated by translocated I-SceI during the Agrobacterium mediated transformation of yeast.
Article in Scientific reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed, 22 citations in OpenAlex.
- Functional dissection ofProceedings of the National Academy of Sciences of the United States of America · 2026Article
- Recruitment of heterologous substrates by bacterial secretion systems for transkingdom translocation.Frontiers in cellular and infection microbiology · 2023Review
- Agrobacterium expressing a type III secretion system delivers Pseudomonas effectors into plant cells to enhance transformation.Nature communications · 2022Article
- Characterization of T-Circles and Their Formation Reveal Similarities toFrontiers in plant science · 2022Article
- Present and future prospects for wheat improvement through genome editing and advanced technologies.Plant communications · 2021Review
- Vigilant: An Engineered VirD2-Cas9 Complex for Lateral Flow Assay-Based Detection of SARS-CoV2.Nano letters · 2021Article
- Fusion of the Cas9 endonuclease and the VirD2 relaxase facilitates homology-directed repair for precise genome engineering in rice.Communications biology · 2020Article
- Precise gene replacement in plants through CRISPR/Cas genome editing technology: current status and future perspectives.aBIOTECH · 2020Review
- CRISPR/Cas9 Mutagenesis by Translocation of Cas9 Protein Into Plant Cells via theFrontiers in genome editing · 2020Article
- CRISPR/Cas-mediated gene targeting in plants: finally a turn for the better for homologous recombination.Plant cell reports · 2019Review
- Combined strategy of transcription factor manipulation and β-glucosidase gene overexpression in Trichoderma reesei and its application in lignocellulose bioconversion.Journal of industrial microbiology & biotechnology · 2018Article
- Constitutive hyperproduction of sorbicillinoids inBiotechnology for biofuels · 2018Article
- The Conjugative Relaxase TrwC Promotes Integration of Foreign DNA in the Human Genome.Applied and environmental microbiology · 2017Article
- Development of an efficient vector system for gene knock-out and near in-cis gene complementation in the sugarcane smut fungus.Scientific reports · 2017Article
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Authors and funding
3 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Agrobacterium mediated transformation (AMT) has been embraced by biotechnologists as the technology of choice to introduce or alter genetic traits of plants. However, in plants it is virtually impossible to predetermine the integration site of the transferred T-strand unless one is able to generate a double stranded break (DSB) in the DNA at the site of interest. In this study, we used the model organism Saccharomyces cerevisiae to investigate whether the Agrobacterium mediated translocation of site-specific endonucleases via the type IV secretion system (T4SS), concomitantly with T-DNA transfer is possible and whether this can improve the gene targeting efficiency. In addition to that, the effect of different chromatin states on targeted integration, was investigated. It was found that Agrobacterium mediated translocation of the homing endonuclease I-SceI has a positive effect on the integration of T-DNA via the homologous repair (HR) pathway. Furthermore, we obtained evidence that nucleosome removal has a positive effect on I-SceI facilitated T-DNA integration by HR. Reversely; inducing nucleosome formation at the site of integration removes the positive effect of translocated I-SceI on T-DNA integration.
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