ArticleStem cell reports2015
In vivo repopulating activity emerges at the onset of hematopoietic specification during embryonic stem cell differentiation.
Article in Stem cell reports, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed.
- Article
- Haematopoietic development and HSC formation in vitro: promise and limitations of gastruloid models.Emerging topics in life sciences · 2023Review
- Using Pluripotent Stem Cells to Understand Normal and Leukemic Hematopoietic Development.Stem cells translational medicine · 2022Review
- Regeneration of immunocompetent B lymphopoiesis from pluripotent stem cells guided by transcription factors.Cellular & molecular immunology · 2022Article
- Scalable in vitro production of defined mouse erythroblasts.PloS one · 2022Article
- Enhanced HSC-like cell generation from mouse pluripotent stem cells in a 3D induction system cocultured with stromal cells.Stem cell research & therapy · 2021Article
- Long Non-coding RNAs and MicroRNAs Interplay in Osteogenic Differentiation of Mesenchymal Stem Cells.Frontiers in cell and developmental biology · 2021Review
- Two-step protocol for regeneration of immunocompetent T cells from mouse pluripotent stem cells.Blood science (Baltimore, Md.) · 2020Article
- Gata3 targets Runx1 in the embryonic haematopoietic stem cell niche.IUBMB life · 2020Article
- Blood making: learning what to put into the dish.F1000Research · 2020Review
- Modeling Leukemia with Human Induced Pluripotent Stem Cells.Cold Spring Harbor perspectives in medicine · 2019Review
- Modeling blood diseases with human induced pluripotent stem cells.Disease models & mechanisms · 2019Review
- Endothelial-to-haematopoietic transition: an update on the process of making blood.Biochemical Society transactions · 2019Review
- Iron deficiency disrupts embryonic haematopoiesis but not the endothelial to haematopoietic transition.Scientific reports · 2019Article
- Long-Term Engraftment of ESC-Derived B-1 Progenitor Cells Supports HSC-Independent Lymphopoiesis.Stem cell reports · 2019Article
- Early Human Hemogenic Endothelium Generates Primitive and Definitive Hematopoiesis In Vitro.Stem cell reports · 2018Article
- Notch and Aryl Hydrocarbon Receptor Signaling Impact Definitive Hematopoiesis from Human Pluripotent Stem Cells.Stem cells (Dayton, Ohio) · 2018Article
- Single-cell transcriptomics reveal the dynamic of haematopoietic stem cell production in the aorta.Nature communications · 2018Article
- HOXB4 Promotes Hemogenic Endothelium Formation without Perturbing Endothelial Cell Development.Stem cell reports · 2018Article
- In Vitro Differentiation of Gata2 and Ly6a Reporter Embryonic Stem Cells Corresponds to In Vivo Waves of Hematopoietic Cell Generation.Stem cell reports · 2018Article
Corrections and comments
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Authors and funding
5 authors.
Funding
Abstract
The generation of in vivo repopulating hematopoietic cells from in vitro differentiating embryonic stem cells has remained a long-standing challenge. To date, hematopoietic engraftment has mostly been achieved through the enforced expression of ectopic transcription factors. Here, we describe serum-free culture conditions that allow the generation of in vivo repopulating hematopoietic cells in the absence of ectopically expressed factors. We show that repopulating activity arises immediately upon the commitment of mesodermal precursors to the blood program, within the first wave of hematopoietic specification. We establish that the formation of these progenitors is extremely transient and exquisitely sensitive to the cytokine milieu. Our findings define the precise differentiating stage at which hematopoietic repopulating activity first appears in vitro, and suggest that during embryonic stem cell differentiation, all hematopoietic programs are unraveled simultaneously from the mesoderm in the absence of cues that restrict the coordinated emergence of each lineage as is normally observed during embryogenesis.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.