ArticlePlacenta2015
Expression pattern and function of Notch2 in different subtypes of first trimester cytotrophoblast.
Article in Placenta, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 18 papers.
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Who cites it
18 citing papers in PubMed, 29 citations in OpenAlex.
- Dissecting placental host-pathogen interactions: Rift Valley fever virus infection in early human trophoblast stem cells.iScience · 2026Article
- In vitro differentiation of macaque extravillous trophoblasts in a low oxygen environment.Placenta · 2025Article
- Development of apical out trophoblast stem cell derived organoids to model early human pregnancy.iScience · 2025Article
- Trophoblast Organoids: Capturing the Complexity of Early Placental Development In Vitro.Organoids · 2024Article
- YAP-mediated trophoblast dysfunction: the common pathway underlying pregnancy complications.Cell communication and signaling : CCS · 2023Review
- Current Strategies of Modeling Human Trophoblast Using Human Pluripotent Stem Cells in vitro.Current protocols · 2023Article
- Laminin switches terminal differentiation fate of human trophoblast stem cells under chemically defined culture conditions.The Journal of biological chemistry · 2023Article
- Transforming growth factor-β signaling governs the differentiation program of extravillous trophoblasts in the developing human placenta.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- WNT and NOTCH signaling in human trophoblast development and differentiation.Cellular and molecular life sciences : CMLS · 2022Review
- In Vitro Model of Human Trophoblast in Early Placentation.Biomedicines · 2022Review
- Implications for preeclampsia: hypoxia-induced Notch promotes trophoblast migration.Reproduction (Cambridge, England) · 2021Article
- Human placenta and trophoblast development: key molecular mechanisms and model systems.Cellular and molecular life sciences : CMLS · 2019Review
- Self-Renewing Trophoblast Organoids Recapitulate the Developmental Program of the Early Human Placenta.Stem cell reports · 2018Article
- The Effect of Scaffold Modulus on the Morphology and Remodeling of Fetal Mesenchymal Stem Cells.Frontiers in physiology · 2018Article
- Regulation of Placental Extravillous Trophoblasts by the Maternal Uterine Environment.Frontiers in immunology · 2018Review
- Hypoxia Directs Human Extravillous Trophoblast Differentiation in a Hypoxia-Inducible Factor-Dependent Manner.The American journal of pathology · 2017Article
- Notch1 controls development of the extravillous trophoblast lineage in the human placenta.Proceedings of the National Academy of Sciences of the United States of America · 2016Article
- Effects of Notch2 and Notch3 on Cell Proliferation and Apoptosis of Trophoblast Cell Lines.International journal of medical sciences · 2015Article
Corrections and comments
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Authors and funding
5 authors at 1 institution in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
introductionNotch signalling has been shown to control cytotrophoblast (CTB) proliferation, differentiation and motility suggesting that the conserved signalling pathway could be critical for human placental development. Since individual Notch receptors have not been elucidated, we herein investigated expression pattern and function of Notch2 in different first trimester trophoblast subpopulations.
methodsLocalisation of Notch2 was analysed in first trimester placental and decidual tissues using immunofluorescence. Notch2 transcript and protein levels were studied by qRT-PCR and Western blotting in proliferative EGF receptor (EGFR)(+) and differentiated HLA-G(+) CTBs, respectively, isolated from early placentae by MACS. CTB migration through fibronectin-coated transwells as well as proliferation (EdU labelling) in floating villous explant cultures and primary CTBs were investigated in the presence of Notch2 siRNAs or specific antibodies blocking Notch2 cleavage.
resultsIn tissue sections Notch2 expression was higher in HLA-G(+) distal cell column trophoblasts (dCCTs) compared to proximal CCTs. Accordingly, expression of Notch2 mRNA and protein were elevated in isolated HLA-G(+) CTBs compared to EGFR(+) CTBs. Notch2 was also detectable in interstitial CTBs as well as in intramural CTBs associated with maternal decidual vessels. Antibody-mediated inhibition of Notch2 signalling did not affect proliferation, but increased migration of SGHPL-5 cells and primary CTBs. Similarly, Notch2 siRNA treatment promoted trophoblast motility. DISCUSSION: Notch2 is present in differentiated cells of the extravillous trophoblast lineage, such as dCCTs, interstitial and intramural CTBs, suggesting diverse roles of the particular receptor. Notch2 signalling, activated by cell-cell contact of neighbouring dCCTs, could attenuate trophoblast migration.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.