ArticlePLoS pathogens2015
The RhoGAP SPIN6 associates with SPL11 and OsRac1 and negatively regulates programmed cell death and innate immunity in rice.
Article in PLoS pathogens, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 60 papers.
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Who cites it
60 citing papers in PubMed, 125 citations in OpenAlex.
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- ApCtf1β-Interacting Target Proteins BDPH1 and BDEUL12 Regulate Pathogenicity in Arthrinium phaeospermum.Molecular plant pathology · 2026Article
- Ubiquitin-Mediated Proteolysis as a Regulator of the Plant Defense-Growth Balance.Plants (Basel, Switzerland) · 2026Review
- Balancing rice blast resistance and growth through suppression of the E3 ubiquitin ligase OsRING80.Plant physiology · 2025Article
- Genome-Wide Identification of the RhoGAP Gene Family and Main Function of OsRhoGAP2 in Seed Germination of Rice by Transcriptome Analysis.Rice (New York, N.Y.) · 2025Article
- The E3 ubiquitin ligase SlATL2 suppresses tomato immunity by promoting SlCSN5a degradation duringHorticulture research · 2025Article
- Lesion Mimic Mutant: An Ideal Genetic Material for Deciphering the Balance Between Plant Immunity and Growth.Rice (New York, N.Y.) · 2025Review
- Ubiquitin Ligase GenePlants (Basel, Switzerland) · 2025Article
- Integrated Transcriptomic and Metabolomic Analyses Reveal the Importance of the Terpenoid, Fatty Acid, and Flavonoid Pathways in Rice Cell Death and Defense.Plants (Basel, Switzerland) · 2025Article
- Phosphorylation of the transcription factor OsNAC29 by OsMAPK3 activates diterpenoid genes to promote rice immunity.The Plant cell · 2024Article
- Rice E3 ubiquitin ligases: From key modulators of host immunity to potential breeding applications.Plant communications · 2024Review
- Deciphering the mechanism of E3 ubiquitin ligases in plant responses to abiotic and biotic stresses and perspectives on PROTACs for crop resistance.Plant biotechnology journal · 2024Review
- NADP-malic Enzyme OsNADP-ME2 Modulates Plant Height Involving in Gibberellin Signaling in Rice.Rice (New York, N.Y.) · 2024Article
- A genome-wide association study of panicle blast resistance toMolecular breeding : new strategies in plant improvement · 2024Article
- The F-box protein OsFBX156 positively regulates rice defence against the blast fungus Magnaporthe oryzae by mediating ubiquitination-dependent degradation of OsHSP71.1.Molecular plant pathology · 2024Article
- The OsCPK17-OsPUB12-OsRLCK176 module regulates immune homeostasis in rice.The Plant cell · 2024Article
- Genome wide association study reveals new genes for resistance to striped stem borer in rice (Frontiers in plant science · 2024Article
- Genetic dissection of monosaccharides contents in rice whole grain using genome-wide association study.The plant genome · 2023Article
- Suppression of Chitin-Triggered Immunity by Plant Fungal Pathogens: A Case Study of the Cucurbit Powdery Mildew FungusJournal of fungi (Basel, Switzerland) · 2023Review
- MEDIATOR SUBUNIT 16 negatively regulates rice immunity by modulating PATHOGENESIS RELATED 3 activity.Plant physiology · 2023Article
Corrections and comments
- Erratum issued
Authors and funding
12 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
The ubiquitin proteasome system in plants plays important roles in plant-microbe interactions and in immune responses to pathogens. We previously demonstrated that the rice U-box E3 ligase SPL11 and its Arabidopsis ortholog PUB13 negatively regulate programmed cell death (PCD) and defense response. However, the components involved in the SPL11/PUB13-mediated PCD and immune signaling pathway remain unknown. In this study, we report that SPL11-interacting Protein 6 (SPIN6) is a Rho GTPase-activating protein (RhoGAP) that interacts with SPL11 in vitro and in vivo. SPL11 ubiquitinates SPIN6 in vitro and degrades SPIN6 in vivo via the 26S proteasome-dependent pathway. Both RNAi silencing in transgenic rice and knockout of Spin6 in a T-DNA insertion mutant lead to PCD and increased resistance to the rice blast pathogen Magnaporthe oryzae and the bacterial blight pathogen Xanthomonas oryzae pv. oryzae. The levels of reactive oxygen species and defense-related gene expression are significantly elevated in both the Spin6 RNAi and mutant plants. Strikingly, SPIN6 interacts with the small GTPase OsRac1, catalyze the GTP-bound OsRac1 into the GDP-bound state in vitro and has GAP activity towards OsRac1 in rice cells. Together, our results demonstrate that the RhoGAP SPIN6 acts as a linkage between a U-box E3 ligase-mediated ubiquitination pathway and a small GTPase-associated defensome system for plant immunity.
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