ArticlePloS one2015
Selection of suitable reference genes for qPCR normalization under abiotic stresses and hormone stimuli in carrot leaves.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 82 papers.
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Who cites it
82 citing papers in PubMed.
- Physiological and molecular investigation of nanosilica mediated drought tolerance in cumin (Cuminum cyminum L.).Scientific reports · 2026Article
- Methyl jasmonate enhances carotenoid accumulation and lignin deposition in carrot taproot.Protoplasma · 2026Article
- Exploring the influence of nanosilica on monoterpene biosynthesis, PAL1 and LS gene expression in cumin (Cuminum cyminum L.) under water-deficit stress.BMC plant biology · 2026Article
- Selection and validation of suitable reference genes for qPCR expression analysis in youngFrontiers in plant science · 2026Article
- Screening and Identification of Reference Genes Under Different Conditions and Growth Stages ofInternational journal of molecular sciences · 2025Article
- Ethylene response factors as potential regulators of cell wall formation in celery (Apium graveolens L.) collenchyma: phylogeny and gene expression analysis.Protoplasma · 2025Article
- Carotene hydroxylase DcCYP97A3 affects carotenoid metabolic flow and taproot color by influencing the conversion of α-carotene to lutein in carrot.Horticulture research · 2025Article
- Integrated metabolome analysis and transcript profiles revealed a potential role of SWEETs in sugar accumulation during Carrot taproot development.BMC plant biology · 2025Article
- DcNCED2 promotes ABA synthesis via carotenoid degradation and enhances drought resistance in carrot.Plant cell reports · 2025Article
- Selection and validation of reference genes in alfalfa based on transcriptome sequence data.Scientific reports · 2025Article
- Identifying Core Genes Related to Low-Temperature Stress Resistance in Quinoa Seedlings Based on WGCNA.International journal of molecular sciences · 2024Article
- Integrated volatile metabolome and transcriptome analyses provide insights into the warm aroma formation elicited by methyl jasmonate in carrot root.Frontiers in plant science · 2024Article
- Grass lignin: biosynthesis, biological roles, and industrial applications.Frontiers in plant science · 2024Review
- Article
- Selection of reference genes for RT-qPCR analysis of rice with Rhizoctonia solani infection and biocontrol PGPR/KSi application.Molecular biology reports · 2023Article
- Selection of suitable reference genes for qPCR normalization in different developmental stages ofFrontiers in plant science · 2023Article
- The genetic control of polyacetylenes involved in bitterness of carrots (Daucus carota L.): Identification of QTLs and candidate genes from the plant fatty acid metabolism.BMC plant biology · 2022Article
- Selection and validation of reference genes for RT-qPCR analysis of different organs at various development stages inOpen life sciences · 2022Article
- Differential hydroxylation efficiency of the two non-heme carotene hydroxylases: DcBCH1, rather than DcBCH2, plays a major role in carrot taproot.Horticulture research · 2022Article
- Targeted transcriptomics reveals signatures of large-scale independent origins and concerted regulation of effector genes in Radopholus similis.PLoS pathogens · 2021Article
22 more citing papers are in PubMed but not listed here.
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Authors and funding
6 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carrot, a biennial herb of the Apiaceae family, is among the most important vegetable crops in the world. In this study, nine candidate reference genes (GAPDH, ACTIN, eIF-4α, PP2A, SAND, TIP41, UBQ, EF-1α, and TUB) were cloned from carrot. Carrot plants were subjected to abiotic stresses (heat, cold, salt, and drought) and hormone stimuli (gibberellin, salicylic acid, methyl jasmonate, and abscisic acid). The expression profiles of the candidate reference genes were evaluated in three technical and biological replicates. Real-time qPCR data analyses were performed using three commonly used Excel-based applets namely, BestKeeper, geNorm, and NormFinder. ACTIN and TUB were the most stable genes identified among all sample groups, but individual analysis revealed changes in their expression profiles. GAPDH displayed the maximum stability for most of single stresses. To further validate the suitability of the reference genes identified in this study, the expression profile of DcDREB-A1 gene (homolog of AtDREB-A1 gene of Arabidophsis) was studied in carrot. The appropriate reference genes were selected that showed stable expression under the different experimental conditions.
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