ArticlePloS one2015
Identification of candidate agents active against N. ceranae infection in honey bees: establishment of a medium throughput screening assay based on N. ceranae infected cultured cells.
Article in PloS one, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 14 papers.
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Who cites it
14 citing papers in PubMed.
- Fall Treatment with Fumagillin Contributes to an Overwinter Shift inLife (Basel, Switzerland) · 2024Article
- Bleomycin reducesMicrobiology spectrum · 2024Article
- Treatment of MicrosporidiumMicroorganisms · 2024Article
- Construction of scFv Antibodies against the Outer Loops of the MicrosporidiumInternational journal of molecular sciences · 2022Article
- Laboratory Cultivation ofInsects · 2022Article
- In Vivo Inhibitory Assessment of Potential Antifungal Agents onPathogens (Basel, Switzerland) · 2022Article
- Natural Substances, Probiotics, and Synthetic Agents in the Treatment and Prevention of Honeybee Nosemosis.Pathogens (Basel, Switzerland) · 2022Review
- Paromomycin ReducesMicroorganisms · 2022Article
- Recent Advances in the Biocontrol of Nosemosis in Honey Bees (Journal of fungi (Basel, Switzerland) · 2022Review
- Nosema apis and N. ceranae Infection in Honey bees: A Model for Host-Pathogen Interactions in Insects.Experientia supplementum (2012) · 2022Article
- Genome and Evolutionary Analysis ofFrontiers in microbiology · 2021Article
- Article
- Bioactivity studies of porphyrinoids against microsporidia isolated from honeybees.Scientific reports · 2020Article
- Scientific Advances in ControllingFrontiers in veterinary science · 2019Review
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2 authors.
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Abstract
Many flowering plants in both natural ecosytems and agriculture are dependent on insect pollination for fruit set and seed production. Managed honey bees (Apis mellifera) and wild bees are key pollinators providing this indispensable eco- and agrosystem service. Like all other organisms, bees are attacked by numerous pathogens and parasites. Nosema apis is a honey bee pathogenic microsporidium which is widely distributed in honey bee populations without causing much harm. Its congener Nosema ceranae was originally described as pathogen of the Eastern honey bee (Apis cerana) but jumped host from A. cerana to A. mellifera about 20 years ago and spilled over from A. mellifera to Bombus spp. quite recently. N. ceranae is now considered a deadly emerging parasite of both Western honey bees and bumblebees. Hence, novel and sustainable treatment strategies against N. ceranae are urgently needed to protect honey and wild bees. We here present the development of an in vitro medium throughput screening assay for the identification of candidate agents active against N. ceranae infections. This novel assay is based on our recently developed cell culture model for N. ceranae and coupled with an RT-PCR-ELISA protocol for quantification of N. ceranae in infected cells. The assay has been adapted to the 96-well microplate format to allow automated analysis. Several substances with known (fumagillin) or presumed (surfactin) or no (paromomycin) activity against N. ceranae were tested as well as substances for which no data concerning N. ceranae inhibition existed. While fumagillin and two nitroimidazoles (metronidazole, tinidazole) totally inhibited N. ceranae proliferation, all other test substances were inactive. In summary, the assay proved suitable for substance screening and demonstrated the activity of two synthetic antibiotics against N. ceranae.
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