ReviewInterface focus2015
Experimental strategies for the identification and characterization of adhesive proteins in animals: a review.
Review in Interface focus, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Who cites it
46 citing papers in PubMed.
- Recombinant Proteins: A Molecular Tool to Understand Marine Adhesion and to Advance Biomaterials.Advanced healthcare materials · 2026Review
- Amyloid-like Structures in Marine Adhesive Proteins.Marine drugs · 2025Review
- Exogenous adenosine promotes barnacle (Amphibalanus amphitrite) cyprid settlement through molecular signaling and improved adhesive mechanics.Communications biology · 2025Article
- Perceiving and Countering Marine Biofouling: Structure, Forces, and Processes at Surfaces in Sea Water Across the Length Scales.Langmuir : the ACS journal of surfaces and colloids · 2025Review
- Phase separation and ageing of glycine-rich protein from tick adhesive.Nature chemistry · 2025Article
- The cement of the tube-dwelling polychaeteBeilstein journal of nanotechnology · 2025Article
- Article
- Immunoinformatics Design of a Multiepitope Vaccine (MEV) TargetingPathogens (Basel, Switzerland) · 2024Article
- Recurrent evolution of adhesive defence systems in amphibians by parallel shifts in gene expression.Nature communications · 2024Article
- Characterization of Morphologically Distinct Components in the Tarsal Secretion ofBiomimetics (Basel, Switzerland) · 2023Article
- Hyposalinity reduces coordination and adhesion of sea urchin tube feet.The Journal of experimental biology · 2023Article
- TheProceedings of the National Academy of Sciences of the United States of America · 2023Article
- Protein-Based Biological Materials: Molecular Design and Artificial Production.Chemical reviews · 2023Review
- High Performance Marine and Terrestrial Bioadhesives and the Biomedical Applications They Have Inspired.Molecules (Basel, Switzerland) · 2022Review
- Identification and characterization of proteins that form the inner core Ixodes scapularis tick attachment cement layer.Scientific reports · 2022Article
- Comparative Genomics Reveals Evolutionary Drivers of Sessile Life and Left-right Shell Asymmetry in Bivalves.Genomics, proteomics & bioinformatics · 2022Article
- The Involvement of Cell-Type-Specific Glycans inBiomimetics (Basel, Switzerland) · 2022Article
- Exploration of sea anemone-inspired high-performance biomaterials with enhanced antioxidant activity.Bioactive materials · 2022Article
- Decoding the byssus fabrication by spatiotemporal secretome analysis of scallop foot.Computational and structural biotechnology journal · 2022Article
- Insight Into Novel Anti-tuberculosis Vaccines by Using Immunoinformatics Approaches.Frontiers in microbiology · 2022Article
Corrections and comments
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Authors and funding
5 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Adhesive secretions occur in both aquatic and terrestrial animals, in which they perform diverse functions. Biological adhesives can therefore be remarkably complex and involve a large range of components with different functions and interactions. However, being mainly protein based, biological adhesives can be characterized by classical molecular methods. This review compiles experimental strategies that were successfully used to identify, characterize and obtain the full-length sequence of adhesive proteins from nine biological models: echinoderms, barnacles, tubeworms, mussels, sticklebacks, slugs, velvet worms, spiders and ticks. A brief description and practical examples are given for a variety of tools used to study adhesive molecules at different levels from genes to secreted proteins. In most studies, proteins, extracted from secreted materials or from adhesive organs, are analysed for the presence of post-translational modifications and submitted to peptide sequencing. The peptide sequences are then used directly for a BLAST search in genomic or transcriptomic databases, or to design degenerate primers to perform RT-PCR, both allowing the recovery of the sequence of the cDNA coding for the investigated protein. These sequences can then be used for functional validation and recombinant production. In recent years, the dual proteomic and transcriptomic approach has emerged as the best way leading to the identification of novel adhesive proteins and retrieval of their complete sequences.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.