ArticleExperimental & translational stroke medicine2015
Platelet rich clots are resistant to lysis by thrombolytic therapy in a rat model of embolic stroke.
Article in Experimental & translational stroke medicine, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 38 papers, 1 of them a synthesis that pooled it.
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Who cites it
38 citing papers in PubMed, 1 synthesis or guideline pooled it.
- Microbubbles combined with ultrasound therapy in ischemic stroke: A systematic review of in-vivo preclinical studies.PloS one · 2018Pooled it
- Thrombolytic resistance: an important factor leading to the failure of intravenous thrombolytic therapy.Journal of neurology · 2026Review
- Von Willebrand factor A1 blockade prevents platelet-mediated sustained occlusion for the treatment of arterial thrombosis.Communications biology · 2026Article
- Cancer-associated stroke: a comparative analysis of thrombus composition, resistance to lysis, and plasma biomarkers.Research and practice in thrombosis and haemostasis · 2026Article
- DNase1 RS1053874 Polymorphism is Associated with Early Neurological Recovery through NET Modulation and with Long-Term Survival in Ischemic Stroke: A Prospective Cohort Study.Annals of neurology · 2026Observational
- Toward improved thrombolysis in ischemic stroke: targeting nonfibrin components.Research and practice in thrombosis and haemostasis · 2026Review
- Revisiting Incomplete Tissue-Level Reperfusion Following Successful Thrombectomy for Acute Ischemic Stroke.Annals of neurology · 2026Review
- Thrombus composition in ischaemic stroke: histological and radiological evaluation, and implications for acute clinical management.Journal of thrombosis and thrombolysis · 2025Review
- Management of vision loss associated with complications of cosmetic filler injections.Frontiers in ophthalmology · 2025Review
- Phthisical eye and orbital ischemia after cosmetic platelet-rich plasma injection to the forehead.American journal of ophthalmology case reports · 2024Article
- Sonothrombolysis for Ischemic Stroke.Journal of cardiovascular development and disease · 2024Review
- A collateral circulation in ischemic stroke accelerates recanalization due to lower clot compaction.PloS one · 2024Article
- The role of neutrophils in tPA thrombolysis after stroke: a malicious troublemaker.Frontiers in immunology · 2024Review
- Sonothrombolysis: State-of-the-Art and Potential Applications in Children.Children (Basel, Switzerland) · 2023Review
- Effects of prior antiplatelet use on futile reperfusion in patients with acute ischemic stroke receiving endovascular treatment.European stroke journal · 2023Article
- Platelet-targeted thrombolysis for treatment of acute ischemic stroke.Blood advances · 2023Article
- All tangled up: interactions of the fibrinolytic and innate immune systems.Frontiers in medicine · 2023Review
- Platelet-Neutrophil Association in NETs-Rich Areas in the Retrieved AIS Patient Thrombi.International journal of molecular sciences · 2022Article
- Development of a carotid artery thrombolysis stroke model in mice.Blood advances · 2022Article
- Composition and Organization of Acute Ischemic Stroke Thrombus: A Wealth of Information for Future Thrombolytic Strategies.Frontiers in neurology · 2022Review
Corrections and comments
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Authors and funding
7 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
backgroundEarly recanalization of occluded vessels in stroke is closely associated with improved clinical outcome. Microbubble-enhanced sonothrombolysis is a promising therapy to improve recanalization rates and reduce the time to recanalization. Testing any thrombolytic therapy requires a model of thromboembolic stroke, but to date these models have been highly variable with regards to clot stability. Here, we developed a model of thromboembolic stroke in rats with site-specific delivery of platelet-rich clots (PRC) to the main stem of the middle cerebral artery (MCA). This model was used in a subsequent study to test microbubble-enhanced sonothrombolysis.
methodsIn Study 1 we investigated spontaneous recanalization rates of PRC in vivo over 4 hours and measured infarct volumes at 24 hours. In Study 2 we investigated tPA-mediated thrombolysis and microbubble-enhanced sonothrombolysis in this model.
resultsStudy 1 demonstrated stable occlusion out to 4 hours in 5 of 7 rats. Two rats spontaneously recanalized at 40 and 70 minutes post-embolism. Infarct volumes were not significantly different in recanalized rats, 43.93 ± 15.44% of the ischemic hemisphere, compared to 48.93 ± 3.9% in non-recanalized animals (p = 0.7). In Study 2, recanalization was not observed in any of the groups post-treatment.
conclusionsSite specific delivery of platelet rich clots to the MCA origin resulted in high rates of MCA occlusion, low rates of spontaneous clot lysis and large infarction. These platelet rich clots were highly resistant to tPA with or without microbubble-enhanced sonothrombolysis. This resistance of platelet rich clots to enhanced thrombolysis may explain recanalization failures clinically and should be an impetus to better clot-type identification and alternative recanalization methods.
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