ArticleFrontiers in plant science2015
Disintegration of microtubules in Arabidopsis thaliana and bladder cancer cells by isothiocyanates.
Article in Frontiers in plant science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 17 papers.
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Who cites it
17 citing papers in PubMed.
- Identification and improvement of isothiocyanate-based inhibitors on stomatal opening to act as drought tolerance-conferring agrochemicals.Nature communications · 2023Article
- The Role of thePlants (Basel, Switzerland) · 2021Article
- Review
- Metabolomics and Physiological Approach to Understand Allelopathic Effect of Horseradish Extract on Onion Root and Lettuce Seed as Model Organism.Plants (Basel, Switzerland) · 2021Article
- Inhibition of light-induced stomatal opening by allyl isothiocyanate does not require guard cell cytosolic Ca2+ signaling.Journal of experimental botany · 2020Article
- Chemical Priming by Isothiocyanates Protects Against Intoxication by Products of the Mustard Oil Bomb.Frontiers in plant science · 2020Article
- The Role of a Glucosinolate-Derived Nitrile in Plant Immune Responses.Frontiers in plant science · 2020Article
- Cruciferous Vegetables, Isothiocyanates, and Bladder Cancer Prevention.Molecular nutrition & food research · 2018Review
- Arabidopsis mutants impaired in glutathione biosynthesis exhibit higher sensitivity towards the glucosinolate hydrolysis product allyl-isothiocyanate.Scientific reports · 2018Article
- Glucosinolate-Derived Isothiocyanates Inhibit Arabidopsis Growth and the Potency Depends on Their Side Chain Structure.International journal of molecular sciences · 2017Article
- Allyl-isothiocyanate treatment induces a complex transcriptional reprogramming including heat stress, oxidative stress and plant defence responses in Arabidopsis thaliana.BMC genomics · 2016Article
- De novo Transcriptome Analysis of Sinapis alba in Revealing the Glucosinolate and Phytochelatin Pathways.Frontiers in plant science · 2016Article
- Allyl Isothiocyanate Inhibits Actin-Dependent Intracellular Transport in Arabidopsis thaliana.International journal of molecular sciences · 2015Article
- Antigenotoxicity and Tumor Growing Inhibition by Leafy Brassica carinata and Sinigrin.Molecules (Basel, Switzerland) · 2015Article
- Allyl isothiocyanate affects the cell cycle of Arabidopsis thaliana.Frontiers in plant science · 2015Article
- Editorial: Physiology and Cellular Mechanisms of Isothiocyanates and Other Glucosinolate Degradation Products in Plants.Frontiers in plant science · 2015Article
- Allyl isothiocyanate depletes glutathione and upregulates expression of glutathione S-transferases in Arabidopsis thaliana.Frontiers in plant science · 2015Article
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4 authors.
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No grant is acknowledged in the PubMed record.
Abstract
Isothiocyanates (ITCs) from biodegradation of glucosinolates comprise a group of electrophiles associated with growth-inhibitory effects in plant- and mammalian cells. The underlying modes of action of this feature are not fully understood. Clarifying this has involved mammalian cancer cells due to ITCs' chemopreventive potential. The binding of ITCs to tubulins has been reported as a mechanism by which ITCs induce cell cycle arrest and apoptosis. In the present study we demonstrate that ITCs disrupt microtubules in Arabidopsis thaliana contributing to the observed inhibited growth phenotype. We also confirmed this in rat bladder cancer cells (AY-27) suggesting that cells from plant and animals share mechanisms by which ITCs affect growth. Exposure of A. thaliana to vapor-phase of allyl ITC (AITC) inhibited growth and induced a concurrent bleaching of leaves in a dose-dependent manner. Transcriptional analysis was used to show an upregulation of heat shock-genes upon AITC-treatment. Transgenic A. thaliana expressing GFP-marked α-tubulin was employed to show a time- and dose-dependent disintegration of microtubules by AITC. Treatment of AY-27 with ITCs resulted in a time- and dose-dependent decrease of cell proliferation and G2/M-arrest. AY-27 transiently transfected to express GFP-tagged α-tubulin were treated with ITCs resulting in a loss of microtubular filaments and the subsequent formation of apoptotic bodies. In conclusion, our data demonstrate an ITC-induced mechanism leading to growth inhibition in A. thaliana and rat bladder cancer cells, and expose clues to the mechanisms underlying the physiological role of glucosinolates in vivo.
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