ArticleMolecular biology of the cell2015
Cell shape impacts on the positioning of the mitotic spindle with respect to the substratum.
Article in Molecular biology of the cell, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. An erratum has been issued. Cited by 13 papers.
What it found
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The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.
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Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.
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Who cites it
13 citing papers in PubMed.
- Mechanical stress combines with planar polarised patterning during metaphase to orient embryonic epithelial cell divisions.Development (Cambridge, England) · 2024Article
- Tension at intercellular junctions is necessary for accurate orientation of cell division in the epithelium plane.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Spindle reorientation in response to mechanical stress is an emergent property of the spindle positioning mechanisms.Proceedings of the National Academy of Sciences of the United States of America · 2022Article
- Agro-Morphological, Microanatomical and Molecular Cytogenetic Characterization of the Medicinal PlantPlants (Basel, Switzerland) · 2020Article
- Mechanics of Multicentrosomal Clustering in Bipolar Mitotic Spindles.Biophysical journal · 2020Article
- Intramolecular interaction in LGN, an adaptor protein that regulates mitotic spindle orientation.The Journal of biological chemistry · 2019Article
- Measurement of Mitotic Spindle Angle and Mitotic Cell Distance in Fixed Tissue ofBio-protocol · 2019Article
- Actomyosin-Driven Tension at Compartmental Boundaries Orients Cell Division Independently of Cell Geometry In Vivo.Developmental cell · 2018Article
- Impact of physical confinement on nuclei geometry and cell division dynamics in 3D spheroids.Scientific reports · 2018Article
- Cell-cell adhesion accounts for the different orientation of columnar and hepatocytic cell divisions.The Journal of cell biology · 2017Article
- 27 T ultra-high static magnetic field changes orientation and morphology of mitotic spindles in human cells.eLife · 2017Article
- Vertebrate Embryonic Cleavage Pattern Determination.Advances in experimental medicine and biology · 2017Review
- Regulation of mitotic spindle orientation: an integrated view.EMBO reports · 2016Review
Corrections and comments
- Erratum issuedCorrection.2015
Authors and funding
3 authors.
Funding
Abstract
All known mechanisms of mitotic spindle orientation rely on astral microtubules. We report that even in the absence of astral microtubules, metaphase spindles in MDCK and HeLa cells are not randomly positioned along their x-z dimension, but preferentially adopt shallow β angles between spindle pole axis and substratum. The nonrandom spindle positioning is due to constraints imposed by the cell cortex in flat cells that drive spindles that are longer and/or wider than the cell's height into a tilted, quasidiagonal x-z position. In rounder cells, which are taller, fewer cortical constraints make the x-z spindle position more random. Reestablishment of astral microtubule-mediated forces align the spindle poles with cortical cues parallel to the substratum in all cells. However, in flat cells, they frequently cause spindle deformations. Similar deformations are apparent when confined spindles rotate from tilted to parallel positions while MDCK cells progress from prometaphase to metaphase. The spindle disruptions cause the engagement of the spindle assembly checkpoint. We propose that cell rounding serves to maintain spindle integrity during its positioning.
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Registered trials
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