ArticlePlant molecular biology2015
Genetic manipulation of a high-affinity PHR1 target cis-element to improve phosphorous uptake in Oryza sativa L.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 30 papers.
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Who cites it
30 citing papers in PubMed, 58 citations in OpenAlex.
- Natural variation inScience advances · 2026Article
- Beyond phosphate uptake: PT4/PT11 as key functional components linking phosphate acquisition, nutrient exchange, and arbuscule maintenance in arbuscular mycorrhizal symbiosis.Frontiers in plant science · 2026Review
- A cis-natural antisense RNA regulates alternative polyadenylation of SlSPX5 under Pi starvation in tomato.Nature communications · 2025Article
- Genome-Wide Identification and Comprehensive Analysis of the GARP Transcription Factor Superfamily inGenes · 2025Article
- Identification and Expression Analysis of Acid Phosphatase Gene (Plants (Basel, Switzerland) · 2025Article
- Genome-Wide Transcriptional Profiling and Functional Analysis Reveal That OsPHT4;4 Is Critical for the Growth and Development of Rice.International journal of molecular sciences · 2024Article
- ZmPHR1 contributes to drought resistance by modulating phosphate homeostasis in maize.Plant biotechnology journal · 2024Article
- MicroRNAs as potent regulators in nitrogen and phosphorus signaling transduction and their applications.Stress biology · 2024Review
- Integrated Analysis of Metabolome and Transcriptome Reveals Insights for Low Phosphorus Tolerance in Wheat Seedling.International journal of molecular sciences · 2023Article
- PHOSPHATE STARVATION RESPONSE1 (PHR1) interacts with JASMONATE ZIM-DOMAIN (JAZ) and MYC2 to modulate phosphate deficiency-induced jasmonate signaling in Arabidopsis.The Plant cell · 2023Article
- Review
- Tissue-specific enhancement of OsRNS1 with root-preferred expression is required for the increase of crop yield.Journal of advanced research · 2022Article
- Transgenic expression of rice OsPHR2 increases phosphorus uptake and yield in wheat.Protoplasma · 2022Article
- Alternative splicing of REGULATOR OF LEAF INCLINATION 1 modulates phosphate starvation signaling and growth in plants.The Plant cell · 2022Article
- Cross-Talk between Transcriptome Analysis and Physiological Characterization Identifies the Genes in Response to the Low Phosphorus Stress inInternational journal of molecular sciences · 2022Article
- Phosphorus Starvation- and Zinc Excess-InducedJournal of fungi (Basel, Switzerland) · 2021Article
- Article
- Identification of transcription factors that bind to the 5'-UTR of the barley PHO2 gene.Plant molecular biology · 2020Article
- Ca14-3-3 Interacts With CaWRKY58 to Positively Modulate Pepper Response to Low-Phosphorus Starvation.Frontiers in plant science · 2020Article
- Phosphate-Starvation-Inducible S-Like RNase Genes in Rice Are Involved in Phosphate Source Recycling by RNA Decay.Frontiers in plant science · 2020Article
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Authors and funding
11 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Phosphorus (P) is an essential macronutrient for crop development and production. Phosphate starvation response 1 (PHR1) acts as the central regulator for Pi-signaling and Pi-homeostasis in plants by binding to the cis-element PHR1 binding sequence (P1BS; GNATATNC). However, how phosphate starvation-induced gene expression is regulated remains obscure. In this work, we investigated the DNA binding affinity of the PHR1 ortholog OsPHR2 to its downstream target genes in Oryza sativa (rice). We confirmed that a combination of P1BS and P1BS-like motifs are essential for stable binding by OsPHR2. Furthermore, we report that variations in P1BS motif bases affected the binding affinity of OsPHR2 and that the highest affinity motif was GaATATtC (designated the A-T-type P1BS). We also found that a combination of two A-T-type P1BS elements in tandem, namely HA-P1BS, was very efficient for binding of OsPHR2. Using the cis-regulator HA-P1BS, we modified the promoters of Transporter Traffic Facilitator 1 (PHF1), a key factor controlling endoplasmic reticulum-exit of phosphate transporters to the plasma membrane, for efficient uptake of phosphorous in an energetically neutral way. Transgenic plants with the modified promoters showed significantly enhanced tolerance to low phosphate stress in both solution and soil conditions, which provides a new strategy for crop improvement to enhance tolerance of nutrient deficiency.
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