ArticlePlant molecular biology2015
A combinatorial bidirectional and bicistronic approach for coordinated multi-gene expression in corn.
Article in Plant molecular biology, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed.
- RUBY-BD: a highly efficient visual reporter system for assessing bidirectional promoter activity.Planta · 2026Article
- Synthetic Biology of Plants and Microbes for Agriculture, Environment, and Future Applications.Chemical reviews · 2026Review
- From Structure to Function of Promoters and 5'UTRs in Maize.International journal of molecular sciences · 2026Review
- Engineering bidirectional chloroplast promoters for tunable co-expression of multiple genes in microalgae (Chlamydomonas reinhardtii).Communications biology · 2026Article
- Advances in promoter engineering strategies for enhanced recombinant protein expression in plants.Frontiers in plant science · 2025Review
- Designing artificial synthetic promoters for accurate, smart, and versatile gene expression in plants.Plant communications · 2023Review
- Review
- Synthetic polycistronic sequences in eukaryotes.Synthetic and systems biotechnology · 2021Review
- Perspectives for epigenetic editing in crops.Transgenic research · 2021Review
- Comprehensive construction strategy of bidirectional green tissue-specific synthetic promoters.Plant biotechnology journal · 2020Article
- TinkeringInternational journal of molecular sciences · 2020Article
- Transcription Terminator-Mediated Enhancement in Transgene Expression in Maize: Preponderance of the AUGAAU Motif Overlapping With Poly(A) Signals.Frontiers in plant science · 2020Article
- Plant Defenses Against Pests Driven by a Bidirectional Promoter.Frontiers in plant science · 2019Article
- Bidirectional Promoter-Based CRISPR-Cas9 Systems for Plant Genome Editing.Frontiers in plant science · 2019Article
- Synthetic Promoters: Designing the cis Regulatory Modules for Controlled Gene Expression.Molecular biotechnology · 2018Review
- Growth rate rather than growth duration drives growth heterosis in maize B104 hybrids.Plant, cell & environment · 2018Article
- Expression of a novel bi-directional Brassica napus promoter in soybean.Transgenic research · 2017Article
- Coordinated protein co-expression in plants by harnessing the synergy between an intein and a viral 2A peptide.Plant biotechnology journal · 2017Article
- Article
- Isolation and Functional Characterization of Bidirectional Promoters in Rice.Frontiers in plant science · 2016Article
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Authors and funding
11 authors.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Transgene stacking in trait development process through genetic engineering is becoming complex with increased number of desired traits and multiple modes of action for each trait. We demonstrate here a novel gene stacking strategy by combining bidirectional promoter (BDP) and bicistronic approaches to drive coordinated expression of multi-genes in corn. A unidirectional promoter, Ubiquitin-1 (ZMUbi1), from Zea mays was first converted into a synthetic BDP, such that a single promoter can direct the expression of two genes from each end of the promoter. The BDP system was then combined with a bicistronic organization of genes at both ends of the promoter by using a Thosea asigna virus 2A auto-cleaving domain. With this gene stacking configuration, we have successfully obtained expression in transgenic corn of four transgenes; three transgenes conferring insect (cry34Ab1 and cry35Ab1) and herbicide (aad1) resistance, and a phiyfp reporter gene using a single ZMUbi1 bidirectional promoter. Gene expression analyses of transgenic corn plants confirmed better coordinated expression of the four genes compared to constructs driving each gene by independent unidirectional ZmUbi1 promoter. To our knowledge, this is the first report that demonstrates application of a single promoter for co-regulation of multiple genes in a crop plant. This stacking technology would be useful for engineering metabolic pathways both for basic and applied research.
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