ArticlePlant biotechnology journal2016
Enhanced waterlogging tolerance in barley by manipulation of expression of the N-end rule pathway E3 ligase PROTEOLYSIS6.
Article in Plant biotechnology journal, 2016. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 70 papers.
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Who cites it
70 citing papers in PubMed, 152 citations in OpenAlex.
- Functional Divergence of the Arg/N-Degron Pathway Between the CropPlant direct · 2026Article
- Interaction Between CsATG8f and CsRAP2.12 Modulates Antioxidant Defense and Hypoxia Response During Submergence inInternational journal of molecular sciences · 2025Article
- Transcriptional signatures associated with waterlogging stress responses and aerenchyma formation in barley root tissue.Annals of botany · 2025Article
- Rainfall patterns during barley seed development underlie genomic variation for germination after flooding.Plant physiology · 2025Article
- Translational insights into abiotic interactions: From Arabidopsis to crop plants.The Plant cell · 2025Review
- Oxygen sensing and plant adaptation to flooding in a changing climate.Philosophical transactions of the Royal Society of London. Series B, Biological sciences · 2025Review
- An mRNA-display derived cyclic peptide scaffold reveals the substrate binding interactions of an N-terminal cysteine oxidase.Nature communications · 2025Article
- The basal level of salicylic acid represses the PRT6 N-degron pathway to modulate root growth and stress response in rice.Plant communications · 2025Article
- Comparative Transcriptome Analyses Reveal the Mechanisms Underlying Waterlogging Tolerance in Barley.Plants (Basel, Switzerland) · 2024Article
- Primed to persevere: Hypoxia regulation from epigenome to protein accumulation in plants.Plant physiology · 2024Review
- Advances in seed hypoxia research.Plant physiology · 2024Article
- Ubiquitin E3 ligases in the plant Arg/N-degron pathway.The Biochemical journal · 2024Review
- New Insight into the Related Candidate Genes and Molecular Regulatory Mechanisms of Waterlogging Tolerance in Tree PeonyPlants (Basel, Switzerland) · 2024Article
- BIG enhances Arg/N-degron pathway-mediated protein degradation to regulate Arabidopsis hypoxia responses and suberin deposition.The Plant cell · 2024Article
- Uncovering waterlogging-responsive genes in cucumber through machine learning and differential gene correlation analysis.Botanical studies · 2024Article
- Tissue distribution of metabolites in Cordyceps cicadae determined by DESI-MSI analysis.Analytical and bioanalytical chemistry · 2024Article
- ERFVII action and modulation through oxygen-sensing in Arabidopsis thaliana.Nature communications · 2023Article
- Transcriptional analysis in multiple barley varieties identifies signatures of waterlogging response.Plant direct · 2023Article
- Genome-Wide Identification and Analysis of the Plant Cysteine Oxidase (PCO) Gene Family inInternational journal of molecular sciences · 2023Article
- Genetic resources and precise gene editing for targeted improvement of barley abiotic stress tolerance.Journal of Zhejiang University. Science. B · 2023Review
10 more citing papers are in PubMed but not listed here.
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Authors and funding
12 authors at 4 institutions in 3 countries.
Funding
Abstract
Increased tolerance of crops to low oxygen (hypoxia) during flooding is a key target for food security. In Arabidopsis thaliana (L.) Heynh., the N-end rule pathway of targeted proteolysis controls plant responses to hypoxia by regulating the stability of group VII ethylene response factor (ERFVII) transcription factors, controlled by the oxidation status of amino terminal (Nt)-cysteine (Cys). Here, we show that the barley (Hordeum vulgare L.) ERFVII BERF1 is a substrate of the N-end rule pathway in vitro. Furthermore, we show that Nt-Cys acts as a sensor for hypoxia in vivo, as the stability of the oxygen-sensor reporter protein MCGGAIL-GUS increased in waterlogged transgenic plants. Transgenic RNAi barley plants, with reduced expression of the N-end rule pathway N-recognin E3 ligase PROTEOLYSIS6 (HvPRT6), showed increased expression of hypoxia-associated genes and altered seed germination phenotypes. In addition, in response to waterlogging, transgenic plants showed sustained biomass, enhanced yield, retention of chlorophyll, and enhanced induction of hypoxia-related genes. HvPRT6 RNAi plants also showed reduced chlorophyll degradation in response to continued darkness, often associated with waterlogged conditions. Barley Targeting Induced Local Lesions IN Genomes (TILLING) lines, containing mutant alleles of HvPRT6, also showed increased expression of hypoxia-related genes and phenotypes similar to RNAi lines. We conclude that the N-end rule pathway represents an important target for plant breeding to enhance tolerance to waterlogging in barley and other cereals.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.