ArticleThe Journal of biological chemistry2015
A new versatile microarray-based method for high throughput screening of carbohydrate-active enzymes.
Article in The Journal of biological chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 28 papers.
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Who cites it
28 citing papers in PubMed, 64 citations in OpenAlex.
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- Structural dissection of two redox proteins from the shipworm symbiont Teredinibacter turnerae.IUCrJ · 2024Article
- Taxonomic and functional stability overrules seasonality in polar benthic microbiomes.The ISME journal · 2024Article
- Viral infection switches the balance between bacterial and eukaryotic recyclers of organic matter during coccolithophore blooms.Nature communications · 2023Article
- Glycoside hydrolase from the GH76 family indicates that marine Salegentibacter sp. Hel_I_6 consumes alpha-mannan from fungi.The ISME journal · 2022Article
- Review
- In silico evaluation of a targeted metaproteomics strategy for broad screening of cellulolytic enzyme capacities in anaerobic microbiome bioreactors.Biotechnology for biofuels and bioproducts · 2022Article
- Synthetic Plant Glycan Microarrays as Tools for Plant Biology.Methods in molecular biology (Clifton, N.J.) · 2022Article
- In situ visualization of glycoside hydrolase family 92 genes in marine flavobacteria.ISME communications · 2021Article
- Removal of eDNA from fabrics using a novel laundry DNase revealed using high-resolution imaging.Scientific reports · 2021Article
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- Diatom fucan polysaccharide precipitates carbon during algal blooms.Nature communications · 2021Article
- Recent Advances in Screening Methods for the Functional Investigation of Lytic Polysaccharide Monooxygenases.Frontiers in chemistry · 2021Review
- Glycan Array Technology.Advances in biochemical engineering/biotechnology · 2021Article
- Article
- On the functional characterization of lytic polysaccharide monooxygenases (LPMOs).Biotechnology for biofuels · 2019Review
- Biphasic cellular adaptations and ecological implications of Alteromonas macleodii degrading a mixture of algal polysaccharides.The ISME journal · 2019Article
- A comprehensive Caenorhabditis elegans N-glycan shotgun array.Glycobiology · 2018Article
- Double blind microarray-based polysaccharide profiling enables parallel identification of uncharacterized polysaccharides and carbohydrate-binding proteins with unknown specificities.Scientific reports · 2018Article
- Branched Pectic Galactan in Phloem-Sieve-Element Cell Walls: Implications for Cell Mechanics.Plant physiology · 2018Article
Corrections and comments
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Authors and funding
9 authors at 5 institutions in 4 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Carbohydrate-active enzymes have multiple biological roles and industrial applications. Advances in genome and transcriptome sequencing together with associated bioinformatics tools have identified vast numbers of putative carbohydrate-degrading and -modifying enzymes including glycoside hydrolases and lytic polysaccharide monooxygenases. However, there is a paucity of methods for rapidly screening the activities of these enzymes. By combining the multiplexing capacity of carbohydrate microarrays with the specificity of molecular probes, we have developed a sensitive, high throughput, and versatile semiquantitative enzyme screening technique that requires low amounts of enzyme and substrate. The method can be used to assess the activities of single enzymes, enzyme mixtures, and crude culture broths against single substrates, substrate mixtures, and biomass samples. Moreover, we show that the technique can be used to analyze both endo-acting and exo-acting glycoside hydrolases, polysaccharide lyases, carbohydrate esterases, and lytic polysaccharide monooxygenases. We demonstrate the potential of the technique by identifying the substrate specificities of purified uncharacterized enzymes and by screening enzyme activities from fungal culture broths.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.