ArticleAsian-Australasian journal of animal sciences2015
AMPKα, C/EBPβ, CPT1β, GPR43, PPARγ, and SCD Gene Expression in Single- and Co-cultured Bovine Satellite Cells and Intramuscular Preadipocytes Treated with Palmitic, Stearic, Oleic, and Linoleic Acid.
Article in Asian-Australasian journal of animal sciences, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 21 papers.
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Who cites it
21 citing papers in PubMed, 37 citations in OpenAlex.
- Up-to-Date Research on the Composition of Blood Slaughterhouse By-Products Derived FBS Substitutes and Their Applicability to Animal Cell Culture.Journal of food science · 2025Article
- Palmitic acid and eicosapentaenoic acid supplementation in 3T3 adipocytes: impact on lipid storage and oxidative stress.Redox report : communications in free radical research · 2024Article
- Effects of altering the ratio of C16:0 and cis-9 C18:1 in rumen bypass fat on growth performance, lipid metabolism, intestinal barrier, cecal microbiota, and inflammation in fattening bulls.Journal of animal science and biotechnology · 2024Article
- miR-383-5p Regulates Preadipocyte Proliferation and Differentiation by TargetingInternational journal of molecular sciences · 2023Article
- Supplementation with rumen-inert fat in the growing phase altered adipogenic gene expression and the size and number of adipocytes in Hanwoo steers.Journal of animal science · 2023Article
- GPR120 Gene expression and activity in subcutaneous and intramuscular adipose tissues of Angus crossbred steers.Journal of animal science · 2023Article
- Productive and physiological responses of feedlot cattle receiving different sources of Ca salts of fatty acids in the finishing diet.Journal of animal science · 2023Article
- Main regulatory factors of marbling level in beef cattle.Veterinary and animal science · 2021Article
- Effects of the association between whole cottonseed and calcium salts of fatty acids on nutrient intake, feedlot performance, and carcass characteristics ofTranslational animal science · 2021Article
- Article
- Effect of ciglitazone on adipogenic transdifferentiation of bovine skeletal muscle satellite cells.Journal of animal science and technology · 2021Article
- Effect of palmitoleic acid on the differentiation of bovine skeletal muscle satellite cells.Journal of animal science and technology · 2021Article
- Evidence for functional G-coupled protein receptors 43 and 120 in subcutaneous and intramuscular adipose tissue of Angus crossbred steers.Journal of animal science · 2021Article
- Calcium salts of fatty acids with varying fatty acid profiles in diets of feedlot-finished Bos indicus bulls: impacts on intake, digestibility, performance, and carcass and meat characteristics.Journal of animal science · 2020Article
- Acetic acid stimulates G-protein-coupled receptor GPR43 and induces intracellular calcium influx in L6 myotube cells.PloS one · 2020Article
- Oleic acid in the absence of a PPARγ agonist increases adipogenic gene expression in bovine muscle satellite cells1.Journal of animal science · 2019Article
- TRIENNIAL GROWTH SYMPOSIUM: THE NUTRITION OF MUSCLE GROWTH: Impacts of nutrition on the proliferation and differentiation of satellite cells in livestock species1,2.Journal of animal science · 2019Review
- Adipogenic/lipogenic gene expression and fatty acid composition in chuck, loin, and round muscles in response to grain feeding of Yanbian Yellow cattle.Journal of animal science · 2018Article
- Carcass and Meat Characteristics and Gene Expression in Intramuscular Adipose Tissue of Korean Native Cattle Fed Finishing Diets Supplemented with 5% Palm Oil.Korean journal for food science of animal resources · 2017Article
- The Expression of Adipogenic Genes in Adipose Tissues of Feedlot Steers Fed Supplementary Palm Oil or Soybean Oil.Asian-Australasian journal of animal sciences · 2016Article
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Authors and funding
8 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We previously demonstrated that bovine subcutaneous preadipocytes promote adipogenic gene expression in muscle satellite cells in a co-culture system. Herein we hypothesize that saturated fatty acids would promote adipogenic/lipogenic gene expression, whereas mono- and polyunsaturated fatty acids would have the opposite effect. Bovine semimembranosus satellite cells (BSC) and intramuscular preadipocytes (IPA) were isolated from crossbred steers and cultured with 10% fetal bovine serum (FBS)/Dulbecco's Modified Eagle Medium (DMEM) and 1% antibiotics during the 3-d proliferation period. After proliferation, cells were treated for 3 d with 3% horse serum/DMEM (BSC) or 5% FBS/DMEM (IPA) with antibiotics. Media also contained 10 μg/mL insulin and 10 μg/mL pioglitazone. Subsequently, differentiating BSC and IPA were cultured in their respective media with 40 μM palmitic, stearic, oleic, or linoleic acid for 4 d. Finally, BSC and IPA were single- or co-cultured for an additional 2 h. All fatty acid treatments increased (p = 0.001) carnitine palmitoyltransferase-1 beta (CPT1β) gene expression, but the increase in CPT1β gene expression was especially pronounced in IPA incubated with palmitic and stearic acid (6- to 17- fold increases). Oleic and linoleic acid decreased (p = 0.001) stearoyl-CoA desaturase (SCD) gene expression over 80% in both BSC and IPA. Conversely, palmitic and stearic acid increased SCD gene expression three fold in co-cultured in IPA, and stearic acid increased AMPKα gene expression in single- and co-cultured BSC and IPA. Consistent with our hypothesis, saturated fatty acids, especially stearic acid, promoted adipogenic and lipogenic gene expression, whereas unsaturated fatty acids decreased expression of those genes associated with fatty acid metabolism.
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