ArticleInvestigative ophthalmology & visual science2015
Rocking media over ex vivo corneas improves this model and allows the study of the effect of proinflammatory cytokines on wound healing.
Article in Investigative ophthalmology & visual science, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 12 papers, 1 of them a synthesis that pooled it.
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Who cites it
12 citing papers in PubMed, 1 synthesis or guideline pooled it, 24 citations in OpenAlex.
- A systematic review of ultrasound biomicroscopy use in pediatric ophthalmology.Eye (London, England) · 2021Pooled it
- Short-term effects of argon cold atmospheric plasma on canine corneasFrontiers in veterinary science · 2025Article
- Exploring interspecies differences inJournal of medical microbiology · 2024Article
- Degradation and Characterisation of Electrospun Polycaprolactone (PCL) and Poly(lactic-co-glycolic acid) (PLGA) Scaffolds for Vascular Tissue Engineering.Materials (Basel, Switzerland) · 2021Article
- Establishment of a Robust and Simple Corneal Organ Culture Model to Monitor Wound Healing.Journal of clinical medicine · 2021Article
- Stem Cell Niche Microenvironment: Review.Bioengineering (Basel, Switzerland) · 2021Review
- Epithelial Regeneration in Human Corneas Preserved in an Active Storage Machine.Translational vision science & technology · 2021Article
- Construction and Evaluation of Collagen-Based Corneal Grafts Using Polycaprolactone To Improve Tension Stress.ACS omega · 2020Article
- Local synthesis of hepcidin in the anterior segment of the eye: A novel observation with physiological and pathological implications.Experimental eye research · 2020Article
- Development of a novel ex vivo equine corneal model.Veterinary ophthalmology · 2017Article
- Ex vivo rabbit and human corneas as models for bacterial and fungal keratitis.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2017Article
- 1,25-dihydroxyvitamin D3 inhibits corneal wound healing in an ex-vivo mouse model.Graefe's archive for clinical and experimental ophthalmology = Albrecht von Graefes Archiv fur klinische und experimentelle Ophthalmologie · 2016Article
Corrections and comments
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Authors and funding
7 authors at 2 institutions in 2 countries.
Funding
Abstract
purposeThe aim of this work was to develop an in vitro cornea model to study the effect of proinflammatory cytokines on wound healing.
methodsInitial studies investigated how to maintain the ex vivo models for up to 4 weeks without loss of epithelium. To study the effect of cytokines, corneas were cultured with the interleukins IL-17A, IL-22, or a combination of IL-17A and IL-22, or lipopolysaccharide (LPS). The effect of IL-17A on wound healing was then examined.
resultsWith static culture conditions, organ cultures deteriorated within 2 weeks. With gentle rocking of media over the corneas and carbon dioxide perfusion, the ex vivo models survived for up to 4 weeks without loss of epithelium. The cytokine that caused the most damage to the cornea was IL-17A. Under static conditions, wound healing of the central corneal epithelium occurred within 9 days, but only a single-layered epithelium formed whether the cornea was exposed to IL-17A or not. With rocking of media gently over the corneas, a multilayered epithelium was achieved 9 days after wounding. In the presence of IL-17A, however, there was no wound healing evident. Characterization of the cells showed that wherever epithelium was present, both differentiated cells and highly proliferative cells were present.
conclusionsWe propose that introducing rocking to extend the effective working life of this model and the introduction of IL-17A to this model to induce aspects of inflammation extend its usefulness to study the effects of agents that influence corneal regeneration under normal and inflamed conditions.
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