Evidence map›Paper›PMID 25655702›Full record

ArticleDevelopment (Cambridge, England)2015

Optical tomography complements light sheet microscopy for in toto imaging of zebrafish development.

Andrea Bassi, Benjamin Schmid, Jan Huisken

Open access · hybridAbstract read
In one paragraph

Article in Development (Cambridge, England), 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 35 papers.

0numbers the graph read from it
0cells of the map it votes in
35citing papers in PubMed
12.4field-weighted citation impact, top 2% of its field
1 · What the graph read from it

What it found

Each row is one number read from the abstract, on the scale the paper reported it, with its interval. Left of the dashed line favours the treatment, right favours the comparator. Under each row is the sentence it came from. New to these charts? A ten-minute tutorial.

The abstract states no effect estimate the extractor could read, or names no intervention and outcome on the map, so this paper lights no cell and moves no belief. It is still indexed, cited and linked below.

2 · The registry

The trial behind it

Trials whose registry record cites this paper, or whose number appears in the abstract. A trial that started after this paper was published is citing it as background, not reporting it.

Neither the registry nor the abstract names a trial number. If this is a trial report, that itself is worth knowing.

3 · Its place in the literature

Who cites it

35 citing papers in PubMed, 78 citations in OpenAlex.

  1. Article
  2. MoSNanomaterials (Basel, Switzerland) · 2025
    Review
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  14. Cerebrovascular development: mechanisms and experimental approaches.Cellular and molecular life sciences : CMLS · 2021
    Review
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  16. Review
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4 · The record

Corrections and comments

PubMed lists nothing against this paper. Absence here is not a guarantee, only a check that was made.

5 · Who and what money

Authors and funding

3 authors at 2 institutions in 2 countries.

Andrea BassiMax Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany Politecnico di Milano, Dipartimento di Fisica, Milano 20133, Italy.
Benjamin SchmidMax Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany.
Jan HuiskenMax Planck Institute of Molecular Cell Biology and Genetics, Dresden 01307, Germany huisken@mpi-cbg.de.
Max Planck Institute of Molecular Cell Biology and Genetics · DEPolitecnico di Milano · IT

Funding

No grant is acknowledged in the PubMed record.

6 · The paper itself

Abstract

Fluorescently labeled structures can be spectrally isolated and imaged at high resolution in living embryos by light sheet microscopy. Multimodal imaging techniques are now needed to put these distinct structures back into the context of the surrounding tissue. We found that the bright-field contrast of unstained specimens in a selective plane illumination microscopy (SPIM) setup can be exploited for in vivo tomographic reconstructions of the three-dimensional anatomy of zebrafish, without causing phototoxicity. We report multimodal imaging of entire zebrafish embryos over several hours of development, as well as segmentation, tracking and automatic registration of individual organs.

Indexed as

AnimalsEmbryo, NonmammalianMicroscopyTomography, OpticalZebrafishFluorescenceLight sheet microscopyOptical tomographySPIMTime-lapse imagingZebrafish

Identifiers

PMID25655702
PMCPMC4352980
OpenAlexW2162226071

What OpenQuestion holds

Textmetadata
LicenceCC BY
Read underepoch 390

Registered trials

None linked

Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.