ArticleNature protocols2015
An optogenetics- and imaging-assisted simultaneous multiple patch-clamp recording system for decoding complex neural circuits.
Article in Nature protocols, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 39 papers.
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Who cites it
39 citing papers in PubMed.
- Toward continuous monitoring systems: emerging trends of on-chip sensors in organ-on-a-chip.Microsystems & nanoengineering · 2026Review
- Article
- Optogenetically enhanced physical reservoir computing withJournal of biomedical optics · 2025Article
- Coding principles and mechanisms of serotonergic transmission modes.Molecular psychiatry · 2025Article
- Sleep prevents brain phosphoproteome disruption to safeguard survival.Cell discovery · 2025Article
- Coding principles of dopaminergic transmission modes.Science advances · 2025Article
- Robotic Fast Patch Clamp in Brain Slices Based on Stepwise Micropipette Navigation and Gigaseal Formation Control.Sensors (Basel, Switzerland) · 2025Article
- Patch-walking, a coordinated multi-pipette patch clamp for efficiently finding synaptic connections.eLife · 2024Article
- Article
- Patch-walking: Coordinated multi-pipette patch clamp for efficiently finding synaptic connections.bioRxiv : the preprint server for biology · 2024Article
- A non-canonical visual cortical-entorhinal pathway contributes to spatial navigation.Nature communications · 2024Article
- A red-emitting carborhodamine for monitoring and measuring membrane potential.Proceedings of the National Academy of Sciences of the United States of America · 2024Article
- Multimodal Nature of the Single-cell Primate Brain Atlas: Morphology, Transcriptome, Electrophysiology, and Connectivity.Neuroscience bulletin · 2024Review
- Immediate reuse of patch-clamp pipettes after ultrasonic cleaning.Scientific reports · 2024Article
- The co-expression of the depolarizing and hyperpolarizing mechanosensitive ion channels in mammalian retinal neurons.Frontiers in medicine · 2024Article
- A red-emitting carborhodamine for monitoring and measuring membrane potential.bioRxiv : the preprint server for biology · 2023Article
- Architectural organization of ∼1,500-neuron modular minicolumnar disinhibitory circuits in healthy and Alzheimer's cortices.Cell reports · 2023Article
- Comparison of fluorescence biosensors and whole-cell patch clamp recording in detecting ACh, NE, and 5-HT.Frontiers in cellular neuroscience · 2023Article
- A biosensing system employing nanowell microelectrode arrays to record the intracellular potential of a single cardiomyocyte.Microsystems & nanoengineering · 2022Article
- Method for Rapid Enzymatic Cleaning for Reuse of Patch Clamp Pipettes: Increasing Throughput by Eliminating Manual Pipette Replacement between Patch Clamp Attempts.Bio-protocol · 2021Article
Corrections and comments
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Authors and funding
10 authors.
Funding
Abstract
Deciphering neuronal circuitry is central to understanding brain function and dysfunction, yet it remains a daunting task. To facilitate the dissection of neuronal circuits, a process requiring functional analysis of synaptic connections and morphological identification of interconnected neurons, we present here a method for stable simultaneous octuple patch-clamp recordings. This method allows physiological analysis of synaptic interconnections among 4-8 simultaneously recorded neurons and/or 10-30 sequentially recorded neurons, and it allows anatomical identification of >85% of recorded interneurons and >99% of recorded principal neurons. We describe how to apply the method to rodent tissue slices; however, it can be used on other model organisms. We also describe the latest refinements and optimizations of mechanics, electronics, optics and software programs that are central to the realization of a combined single- and two-photon microscopy-based, optogenetics- and imaging-assisted, stable, simultaneous quadruple-viguple patch-clamp recording system. Setting up the system, from the beginning of instrument assembly and software installation to full operation, can be completed in 3-4 d.
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