ArticleAnalytical chemistry2015
Quantitative chemoproteomics for site-specific analysis of protein alkylation by 4-hydroxy-2-nonenal in cells.
Article in Analytical chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 46 papers.
What it found
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Who cites it
46 citing papers in PubMed, 96 citations in OpenAlex.
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- Only time will tell: Modelling the kinetics of covalent inhibitors.British journal of pharmacology · 2026Review
- Spatiotemporal profiling of modification-specific proteome secretion uncovers an itaconation-activated tyrosine kinase.Nature communications · 2025Article
- Protein Haptenation and Its Role in Allergy.Chemical research in toxicology · 2024Review
- Functionalizing tandem mass tags for streamlining click-based quantitative chemoproteomics.Communications chemistry · 2024Article
- Lipid Peroxidation and Antioxidant Protection.Biomolecules · 2023Review
- Z-REX: shepherding reactive electrophiles to specific proteins expressed tissue specifically or ubiquitously, and recording the resultant functional electrophile-induced redox responses in larval fish.Nature protocols · 2023Review
- Biochemical Control of the Mitochondrial Protein MitoNEET by Biological Thiols and Lipid-derived Electrophiles.Advances in redox research · 2023Article
- The 4-Hydroxynonenal-Protein Adducts and Their Biological Relevance: Are Some Proteins Preferred Targets?Antioxidants (Basel, Switzerland) · 2023Review
- Overview of Adductomics in Toxicology.Current protocols · 2023Article
- Quantitative profiling of PTM stoichiometry by resolvable mass tags.RSC chemical biology · 2022Article
- A modification-centric assessment tool for the performance of chemoproteomic probes.Nature chemical biology · 2022Article
- Profiling of Protein Carbonylations in Ferroptosis by Chemical Proteomics.Methods in molecular biology (Clifton, N.J.) · 2022Article
- A Paal-Knorr agent for chemoproteomic profiling of targets of isoketals in cells.Chemical science · 2021Article
- Chemoproteomic profiling reveals cellular targets of nitro-fatty acids.Redox biology · 2021Article
- Protein Carbonylation: Emerging Roles in Plant Redox Biology and Future Prospects.Plants (Basel, Switzerland) · 2021Review
- Lipid peroxidation derived reactive carbonyl species in free and conjugated forms as an index of lipid peroxidation: limits and perspectives.Redox biology · 2021Article
- In-Depth AGE and ALE Profiling of Human Albumin in Heart Failure: Ex Vivo Studies.Antioxidants (Basel, Switzerland) · 2021Article
- Currently Available Strategies for Target Identification of Bioactive Natural Products.Frontiers in chemistry · 2021Review
- Dienone Compounds: Targets and Pharmacological Responses.Journal of medicinal chemistry · 2020Article
Corrections and comments
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Authors and funding
4 authors at 1 institution in 1 country.
Funding
Abstract
Protein alkylation by 4-hydroxy-2-nonenal (HNE), an endogenous lipid derived electrophile, contributes to stress signaling and cellular toxicity. Although previous work has identified protein targets for HNE alkylation, the sequence specificity of alkylation and dynamics in a cellular context remain largely unexplored. We developed a new quantitative chemoproteomic platform, which uses isotopically tagged, photocleavable azido-biotin reagents to selectively capture and quantify the cellular targets labeled by the alkynyl analogue of HNE (aHNE). Our analyses site-specifically identified and quantified 398 aHNE protein alkylation events (386 cysteine sites and 12 histidine sites) in intact cells. This data set expands by at least an order of magnitude the number of such modification sites previously reported. Although adducts formed by Michael addition are thought to be largely irreversible, we found that most aHNE modifications are lost rapidly in situ. Moreover, aHNE adduct turnover occurs only in intact cells and loss rates are site-selective. This quantitative chemoproteomics platform provides a versatile general approach to map bioorthogonal-chemically engineered post-translational modifications and their cellular dynamics in a site-specific and unbiased manner.
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Registered trials
Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.