ArticleBioMed research international2015
RCCS bioreactor-based modelled microgravity induces significant changes on in vitro 3D neuroglial cell cultures.
Article in BioMed research international, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 20 papers.
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Who cites it
20 citing papers in PubMed, 40 citations in OpenAlex.
- Therapeutic Role of Probiotics in Gut-Brain Axis Under Microgravity.Indian journal of microbiology · 2025Review
- Isoform-specific vs. pan-histone deacetylase inhibition as approaches for countering glioblastoma: anFrontiers in oncology · 2025Article
- The Discovery of a Specific CKIP-1 Ligand for the Potential Treatment of Disuse Osteoporosis.International journal of molecular sciences · 2024Article
- Simulated Microgravity-Induced Changes to Drug Response in Cancer Cells Quantified Using Fluorescence Morphometry.Life (Basel, Switzerland) · 2023Article
- 3D tumor explant as a novel platform to investigate therapeutic pathways and predictive biomarkers in cancer patients.Frontiers in immunology · 2022Article
- Propagation of Dental and Respiratory Cells and Organs in Microgravity.Journal of visualized experiments : JoVE · 2021Article
- The Rotary Cell Culture System increases NTRK3 expression and promotes neuronal differentiation and migratory ability of neural stem cells cultured on collagen sponge.Stem cell research & therapy · 2021Article
- Synergistic effects of Indian hedgehog and sonic hedgehog on chondrogenesis during cartilage repair.Journal of molecular histology · 2021Article
- Exosomes derived from umbilical cord mesenchymal stem cells in mechanical environment show improved osteochondral activity via upregulation of LncRNA H19.Journal of orthopaedic translation · 2021Article
- Effect of simulated microgravity on metabolism of HGC-27 gastric cancer cells.Oncology letters · 2020Article
- Combined Treatment with Three Natural Antioxidants Enhances Neuroprotection in a SH-SY5Y 3D Culture Model.Antioxidants (Basel, Switzerland) · 2019Article
- Sonic hedgehog promotes chondrogenesis of rabbit bone marrow stem cells in a rotary cell culture system.BMC developmental biology · 2019Article
- Chondrogenic differentiation of bone marrow-derived mesenchymal stem cells following transfection with Indian hedgehog and sonic hedgehog using a rotary cell culture system.Cellular & molecular biology letters · 2019Article
- The subsequent biological effects of simulated microgravity on endothelial cell growth in HUVECs.Chinese journal of traumatology = Zhonghua chuang shang za zhi · 2018Article
- Metabolic Reprogramming and the Recovery of Physiological Functionality in 3D Cultures in Micro-Bioreactors.Bioengineering (Basel, Switzerland) · 2018Article
- Article
- Scaffold-free and scaffold-assisted 3D culture enhances differentiation of bone marrow stromal cells.In vitro cellular & developmental biology. Animal · 2016Article
- Integrated Biophysical and Biochemical Signals Augment Megakaryopoiesis and Thrombopoiesis in a Three-Dimensional Rotary Culture System.Stem cells translational medicine · 2016Article
- Optimization of a 3D Dynamic Culturing System for In Vitro Modeling of Frontotemporal Neurodegeneration-Relevant Pathologic Features.Frontiers in aging neuroscience · 2016Article
- Article
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Authors and funding
6 authors at 2 institutions in 1 country.
Funding
No grant is acknowledged in the PubMed record.
Abstract
We propose a human-derived neuro-/glial cell three-dimensional in vitro model to investigate the effects of microgravity on cell-cell interactions. A rotary cell-culture system (RCCS) bioreactor was used to generate a modelled microgravity environment, and morphofunctional features of glial-like GL15 and neuronal-like SH-SY5Y cells in three-dimensional individual cultures (monotypic aggregates) and cocultures (heterotypic aggregates) were analysed. Cell survival was maintained within all cell aggregates over 2 weeks of culture. Moreover, compared to cells as traditional static monolayers, cell aggregates cultured under modelled microgravity showed increased expression of specific differentiation markers (e.g., GL15 cells: GFAP, S100B; SH-SY5Y cells: GAP43) and modulation of functional cell-cell interactions (e.g., N-CAM and Cx43 expression and localisation). In conclusion, this culture model opens a wide range of specific investigations at the molecular, biochemical, and morphological levels, and it represents an important tool for in vitro studies into dynamic interactions and responses of nervous system cell components to microgravity environmental conditions.
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Read under generation 80e0d062 · epoch 390. Bibliography from PubMed, PubMed Central and OpenAlex; grants from NIH RePORTER; trial links from ClinicalTrials.gov; estimates, votes and beliefs from the OpenQuestion graph.