ArticleFrontiers in chemistry2015
Down-regulation of Fusarium oxysporum endogenous genes by Host-Delivered RNA interference enhances disease resistance.
Article in Frontiers in chemistry, 2015. The graph could read no effect estimate from its abstract, so it casts no vote on the map. Cited by 26 papers.
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Who cites it
26 citing papers in PubMed, 87 citations in OpenAlex.
- Groundbreaking Technologies and the Biocontrol of Fungal Vascular Plant Pathogens.Journal of fungi (Basel, Switzerland) · 2025Review
- Harnessing RNA interference for the control of Fusarium species: A critical review.Molecular plant pathology · 2024Review
- The RNAi Machinery in the FungusNon-coding RNA · 2024Article
- Ectopic expression of a truncated NLR gene from wildFrontiers in plant science · 2024Article
- Strategies for Controlling the Sporulation inJournal of fungi (Basel, Switzerland) · 2022Review
- Small RNA-based plant protection against diseases.Frontiers in plant science · 2022Review
- Concepts and considerations for enhancing RNAi efficiency in phytopathogenic fungi for RNAi-based crop protection using nanocarrier-mediated dsRNA delivery systems.Frontiers in fungal biology · 2022Review
- Endophytic fungi from kale (Frontiers in microbiology · 2022Article
- Host induced gene silencing ofFrontiers in plant science · 2022Article
- Sclerotinia sclerotiorum Thioredoxin1 (SsTrx1) is required for pathogenicity and oxidative stress tolerance.Molecular plant pathology · 2021Article
- RNA Interference Strategies for Future Management of Plant Pathogenic Fungi: Prospects and Challenges.Plants (Basel, Switzerland) · 2021Article
- Threat at One End of the Plant: What Travels to Inform the Other Parts?International journal of molecular sciences · 2021Review
- Review
- Pathogenomics and Management ofPathogens (Basel, Switzerland) · 2020Review
- Movement of small RNAs in and between plants and fungi.Molecular plant pathology · 2020Review
- Study on the efficiency of dsRNAs with increasing length in RNA-based silencing of theRNA biology · 2020Article
- Review
- RNA-Spray-Mediated Silencing ofFrontiers in plant science · 2020Article
- SIGS vs HIGS: a study on the efficacy of two dsRNA delivery strategies to silence Fusarium FgCYP51 genes in infected host and non-host plants.Molecular plant pathology · 2019Article
- Review
Corrections and comments
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Authors and funding
5 authors at 3 institutions in 2 countries.
Funding
No grant is acknowledged in the PubMed record.
Abstract
Fusarium oxysporum is a devastating pathogen causing extensive yield losses in a variety of crops and development of sustainable, environmentally friendly methods to improve crop resistance is crucial. We have used Host-Delivered RNA interference (HD-RNAi) technology to partially silence three different genes (FOW2, FRP1, and OPR) in the hemi-biotrophic fungus F. oxysporum f. sp. conglutinans. Expression of double stranded RNA (dsRNA) molecules targeting fungal pathogen genes was achieved in a number of transgenic Arabidopsis lines. F. oxysporum infecting the transgenic lines displayed substantially reduced mRNA levels on all three targeted genes, with an average of 75, 83, and 72% reduction for FOW2, FRP1, and OPR, respectively. The silencing of pathogen genes had a clear positive effect on the ability of the transgenic lines to fight infection. All transgenic lines displayed enhanced resistance to F. oxysporum with delayed disease symptom development, especially FRP1 and OPR lines. Survival rates after fungal infection were higher in the transgenic lines compared to control wild type plants which consistently showed survival rates of 10%, with FOW2 lines showing 25% survival; FRP1 lines 30-50% survival and OPR between 45 and 70% survival. The down-regulation effect was specific for the targeted genes without unintended effects in related genes. In addition to producing resistant crops, HD-RNAi can provide a useful tool to rapidly screen candidate fungal pathogenicity genes without the need to produce fungal knockout mutants.
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